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首页> 外文期刊>Journal of Experimental Marine Biology and Ecology >Molecular and cellular response to short-term oxygen variations in the Pacific oyster Crassostrea gigas
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Molecular and cellular response to short-term oxygen variations in the Pacific oyster Crassostrea gigas

机译：太平洋牡蛎Crassostrea gigas对短期氧气变化的分子和细胞响应

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摘要

Intertidal organisms have to face daily high oxygen variations from hypoxic to hyperoxic conditions. To cope with such constraints, these organisms must have well developed compensatory mechanisms involving an tioxidant and energetic adjustments. The oyster Crassostrea gigas is regularly exposed to high oxygen fluctu ations and is a good model to understand these physiological regulations. This study aimed to explore the mechanisms involved in response to short-term oxygen variations in the digestive gland and hemocytes of C gigas. The effects of returning into water after an emersion period were tested for 24 h at two different ox ygen concentrations (normoxic 8.8 mg O_2 L~(-1) and hypoxic 2.6 mg O_2 L~(-1)). Gene expression on several anti oxidant enzymes was performed. In addition, a marker of oxidative damage, the malondialdehyde (MDA) was quantified. Hemocyte parameters measured were viability, concentration of different cellular sub populations and oxidative activity. Changes in energetic metabolism were investigated measuring mRNA levels of pyruvate kinase (PK) and phosphoenolpyruvate carboxykinase (PEPCK). Moreover, mRNA levels of the transcription factor HIFα were analyzed. Higher levels of the alternative oxidase (AOX) mRNA in nor moxic conditions corroborate the hypothesis of the AOX having a role in the redox balance. MDA levels did not show any condition effect, suggesting that hypoxic conditions did not cause oxidative stress. mRNA levels of antioxidant enzymes and hemocyte oxidative activity increased during the 24 h of immersion. This sug gests that the disruption of tidal cycles by maintaining oysters immersed resulted in an increase of oxygen-dependent activities. No clear hypoxic responses were detected neither in a switch of PEPCK and PK toward anaerobic metabolism, nor in HIFa regulation. PK mRNA levels were significantly higher in nomoxic conditions, suggesting that more elevated oxygen concentrations would lead to higher energetic metabolism. Hemocyte concentrations were higher in hypoxic conditions suggesting an increased hemato poiesis. This study brings new insights in understanding how oysters, and more in general intertidal mol luscs, cope with short-term oxygen variations.
机译：从低氧到高氧条件，潮间生物每天都要面对高氧变化。为了应对这种限制，这些生物必须具有完善的补偿机制，其中涉及抗氧化剂和能量调节。牡蛎Crassostrea gigas经常暴露于高氧波动下，是了解这些生理规律的好模型。这项研究的目的是探讨吉加氏菌消化腺和血细胞对短期氧气变化的响应所涉及的机制。在两个不同的氧气浓度下（正常氧8.8 mg O_2 L〜（-1）和低氧2.6 mg O_2 L〜（-1））测试出苗期后回到水中的效果24小时。在几种抗氧化酶上进行基因表达。另外，量化了氧化损伤的标志物丙二醛（MDA）。测得的血细胞参数为活力，不同细胞亚群的浓度和氧化活性。通过测量丙酮酸激酶（PK）和磷酸烯醇丙酮酸羧激酶（PEPCK）的mRNA水平来研究能量代谢的变化。此外，分析了转录因子HIFα的mRNA水平。在无氧条件下，较高水平的替代氧化酶（AOX）mRNA证实了AOX在氧化还原平衡中起作用的假说。 MDA水平未显示任何条件影响，表明低氧条件不会引起氧化应激。在浸泡24小时后，抗​​氧化酶的mRNA水平和血细胞氧化活性增加。这表明，通过保持牡蛎浸没来破坏潮汐循环会导致氧依赖性活动的增加。在PEPCK和PK向厌氧代谢的转换以及HIFa调节中均未检测到明显的低氧反应。在诺莫昔条件下，PK mRNA水平显着升高，表明更高的氧气浓度将导致更高的能量代谢。低氧条件下血细胞浓度较高，提示血细胞生成增多。这项研究为了解牡蛎以及一般潮间带软体动物如何应对短期氧气变化带来了新见解。

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    Rossana Sussarellu; Caroline Fabioux; Miguel Camacho Sanchez; Nelly Le Goiec; Christophe Lambert; Philippe Soudant; Dario Moraga;

  • 作者单位

    UMR CNRS 6539-LEMAR (Laboratoire des Sciences de I'Environnement Marin), IUEM (Institut Universitaire Europeen de la Mer), Universite de Bretagne Occidental,Technopdle Brest Iroise, 29280 Plouzani, France;

    UMR CNRS 6539-LEMAR (Laboratoire des Sciences de I'Environnement Marin), IUEM (Institut Universitaire Europeen de la Mer), Universite de Bretagne Occidental,Technopdle Brest Iroise, 29280 Plouzani, France;

    UMR CNRS 6539-LEMAR (Laboratoire des Sciences de I'Environnement Marin), IUEM (Institut Universitaire Europeen de la Mer), Universite de Bretagne Occidental,Technopdle Brest Iroise, 29280 Plouzani, France;

    UMR CNRS 6539-LEMAR (Laboratoire des Sciences de I'Environnement Marin), IUEM (Institut Universitaire Europeen de la Mer), Universite de Bretagne Occidental,Technopdle Brest Iroise, 29280 Plouzani, France;

    UMR CNRS 6539-LEMAR (Laboratoire des Sciences de I'Environnement Marin), IUEM (Institut Universitaire Europeen de la Mer), Universite de Bretagne Occidental,Technopdle Brest Iroise, 29280 Plouzani, France;

    UMR CNRS 6539-LEMAR (Laboratoire des Sciences de I'Environnement Marin), IUEM (Institut Universitaire Europeen de la Mer), Universite de Bretagne Occidental,Technopdle Brest Iroise, 29280 Plouzani, France;

    UMR CNRS 6539-LEMAR (Laboratoire des Sciences de I'Environnement Marin), IUEM (Institut Universitaire Europeen de la Mer), Universite de Bretagne Occidental,Technopdle Brest Iroise, 29280 Plouzani, France;

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  • 正文语种 eng
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  • 关键词

    gene expression; hemocytes; hypoxia; oxidative stress; oyster; re-oxygenation;

    机译：基因表达;血细胞缺氧氧化应激牡蛎;再充氧;

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