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首页> 外文期刊>Journal of Experimental Marine Biology and Ecology >Impact of mesozooplankton grazing on the microbial community revealed by denaturing gradient gel electrophoresis (DGGE)
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Impact of mesozooplankton grazing on the microbial community revealed by denaturing gradient gel electrophoresis (DGGE)

机译：变性梯度凝胶电泳（DGGE）揭示了中生浮游动物对微生物群落的影响

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摘要

Although grazer inclusion incubation experiments are commonly used to evaluate mesozooplankton grazing mortality of phytoplankton, few studies have employed molecular methods to examine the changes in the composition of the microbial community across different grazing treatments. We used denaturing gradient gel electrophoresis (DGGE) to study the impact of mesozooplankton-mediated trophic cascades on microbial food web components at two subtropical coastal sites with contrasting trophic conditions. Reciprocal changes in microbial food web components resulting from trophic cascades were observed, with decreases in >3μm eukaryotes, increases in <3μm eukaryotes and decreases in bacterioplankton. At the estuarine station, diatoms and dinoflagellates accounted for the majority of the micro and nanoplankton, and they, particularly dinoflagellates, were selectively grazed by mesozooplankton. Although the total band numbers of picoeukaryotes did not vary significantly, the relative abundance of different phylogenetic groups changed with the appearance of mesozooplankton grazers. Both total band brightness and species richness and diversity of bacterioplankton declined sharply under mesozooplankton grazing at the coastal site, but not at the estuarine site, suggesting that mesozooplankton-mediated trophic cascade effect was less pronounced in eutrophic estuarine waters. We concluded that DGGE could be a useful tool to track the community shifts of microbial food web in experimental settings and we have demonstrated in this study such shifts in both eukaryotic and prokaryotic microbial communities induced by mesozooplankton grazing. The magnitude of mesozooplankton-mediated trophic cascade effect transferred to picoplankton appears to be stronger at the mesotrophic coastal water than at the eutrophic estuarine water.
机译：尽管通常使用掠食性动物包涵体孵育实验来评估浮游植物的中游浮游动物的放牧死亡率，但很少有研究采用分子方法来研究不同放牧处理过程中微生物群落组成的变化。我们使用变性梯度凝胶电泳（DGGE）研究了中营养浮游生物介导的营养级联对营养条件相反的两个亚热带沿海地区微生物食物网成分的影响。营养级联反应导致微生物食物网成分的相互变化，>3μm真核生物减少，<3μm真核生物增加，浮游细菌减少。在河口站，硅藻和鞭毛藻占微浮游生物和纳米浮游生物的大部分，它们，特别是鞭毛藻，被中游浮游动物选择性放牧。尽管微核真核生物的总条带数量没有显着变化，但不同的系统发生群体的相对丰度随着中微浮游生物放牧者的出现而改变。食盐性浮游动物在沿海站点放牧，但在河口站点放牧的总带亮度和物种丰富度以及浮游生物的多样性均急剧下降，这表明在富营养化河口水域中游浮游动物介导的营养级联效应不太明显。我们得出的结论是，DGGE可能是追踪实验环境中微生物食物网的群落变化的有用工具，并且我们在本研究中证明了中食浮游动物放牧诱导的真核和原核微生物群落的这种变化。中游浮游动物介导的中游浮游生物介导的营养级联效应的强度似乎比富营养化的河口水要强。

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    Hongmei Jing; Hongbin Liu; Thomas Wong; Mianrun Chen;

  • 作者单位

    Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China;

    Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China Division of Environment, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China;

    Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China;

    Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China;

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  • 关键词

    DGGE; grazing; mesozooplankton; microbial food web;

    机译：DGGE;放牧;中层浮游生物;微生物食物网;

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