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首页> 外文期刊>Journal of Experimental Marine Biology and Ecology >Effects of simulated light regimes on gene expression in Antarctic krill (Euphausia superba Dana)
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Effects of simulated light regimes on gene expression in Antarctic krill (Euphausia superba Dana)

机译：模拟光照方案对南极磷虾（Euphausia superba Dana）基因表达的影响

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摘要

A change in photoperiod has been implicated in triggering a transition from an active to a quiescent state in Antarctic krill. We examined this process at the molecular level, to identify processes that are affected when passing a photoperiodic threshold. Antarctic krill captured in the austral autumn were divided into two groups and immediately incubated either under a photoperiod of 12h light:12h darkness (LD), simulating the natural light cycle, or in continuous darkness (DD), simulating winter. All other conditions were kept identical between incubations. After 7 days of adaptation, krill were sampled every 4h over a 24 h period and frozen. Total RNA was extracted from the heads and pooled to construct a suppression subtractive hybridisation library. Differentially expressed sequences were identified and annotated into functional categories through database sequence matching. We found a difference in gene expression between LD and DD krill, with LD krill expressing more genes involved in functions such as metabolism, motor activity, protein binding and various other cellular activities. Eleven of these genes were examined further with quantitative polymerase chain reaction analyses, which revealed that expression levels were significantly higher in LD krill. The genes affected by simulated photoperiodic change are consistent with known features of quiescence, such as a slowing of moult rate, a lowering of activity levels and a reduction in metabolic rate. The expression of proteases involved in apolysis, where the old cuticle separates from the epidermis, showed particular sensitivity to photoperiod and point to the mechanism by which moult rate is adjusted seasonally. Our results show that key processes are already responding at the molecular level after just 7 days of exposure to a changed photoperiodic cycle. We propose that krill switch rapidly between active and quiescent states and that the photoperiodic cycle plays a key role in this process.
机译：光周期的变化与触发南极磷虾从活动状态到静止状态的转变有关。我们在分子水平上检查了该过程，以确定通过光周期阈值时受影响的过程。在南方秋季捕获的南极磷虾分为两组，并在模拟自然光周期的12h光照：12h黑暗（LD）的光照下或模拟冬天的连续黑暗（DD）的条件下立即孵育。孵育之间所有其他条件保持相同。适应7天后，在24小时内每4小时取样一次磷虾并冷冻。从头部提取总RNA并合并以构建抑制性消减杂交文库。鉴定出差异表达的序列，并通过数据库序列匹配将其注释为功能类别。我们发现LD磷虾和DD磷虾之间的基因表达存在差异，其中LD磷虾表达更多的基因，这些基因参与了新陈代谢，运动活动，蛋白质结合和其他各种细胞活动。通过定量聚合酶链反应分析进一步检查了其中的11个基因，结果表明LD磷虾的表达水平明显更高。受模拟的光周期变化影响的基因与已知的静止特征一致，例如蜕皮速度减慢，活性水平降低和代谢速率降低。蛋白酶的表达与解离有关，其中旧表皮与表皮分离，显示出对光周期的特别敏感性，并指出了季节性改变蜕皮率的机制。我们的结果表明，暴露于变化的光周期后仅7天，关键过程已经在分子水平响应。我们建议磷虾在活动状态和静止状态之间快速切换，并且光周期在该过程中起关键作用。

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