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首页> 外文期刊>Journal of Experimental Marine Biology and Ecology >Influence of yeast quality on performance of gnotobiotically grown Artemia
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Influence of yeast quality on performance of gnotobiotically grown Artemia

机译：酵母质量对成瘤生长的卤虫生产性能的影响

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摘要

Using axenically grown Artemia, a model system was developed to evaluate the effect of bacteria on the survival and development of this crustacean. Two strains of baker's yeast (Saccharomyces cerevisiae) were used in all experiments as feed for Artemia: a wild-type strain and its mnn9 mutant, defective in the synthesis of mannoproteins in the outer cell wall. The genetic background, yeast growth phase and growth medium appeared to be important parameters determining the quality of yeast cells as feed for Artemia. A strong positive correlation between Artemia performance and the yeast cell wall chitin and glucan content was obtained, while the mannoprotein content was negatively correlated. Mnn9 yeast cells grown till exponential phase in minimal medium proved to be excellent feed for Artemia, yielding an average 95% survival and 4-mm growth after 6 days at 28 ℃, which is comparable to the best results obtained with algal feed. The standard growth test yields highly reproducible results and can become an excellent tool to study the mode of action of bacteria. Furthermore, yeast cell viability and the method used to kill/sterilize the cells are important parameters influencing nauplii performance.
机译：使用轴生生长的卤虫，开发了一个模型系统来评估细菌对该甲壳动物的存活和发育的影响。在所有实验中，使用两种酵母面包酵母（Saccharomyces cerevisiae）作为卤虫的饲料：一种野生型菌株及其mnn9突变体，在细胞外壁中的甘露糖蛋白合成方面存在缺陷。遗传背景，酵母生长期和生长培养基似乎是决定用作卤虫饲料的酵母细胞质量的重要参数。获得的Artemia性能与酵母细胞壁的几丁质和葡聚糖含量之间具有很强的正相关性，而甘露糖蛋白含量则呈负相关。在基本培养基中生长到指数期的Mnn9酵母细胞被证明是卤虫的极好饲料，在28℃6天后平均存活率达95％，生长4毫米，这与藻类饲料获得的最佳结果相当。标准生长测试可产生高度可重复的结果，可以成为研究细菌作用方式的出色工具。此外，酵母细胞的存活力和用于杀死/灭菌细胞的方法是影响幼体发育的重要参数。

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