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首页> 外文期刊>Journal of Experimental Marine Biology and Ecology >Effect of experimental thermal stress on lipidomes of the soft coral Sinularia sp. and its symbiotic dinoflagellates
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Effect of experimental thermal stress on lipidomes of the soft coral Sinularia sp. and its symbiotic dinoflagellates

机译：实验热应力对软珊瑚Sinularia sp。脂质体的影响。及其共生的鞭毛虫

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A complete or partial loss of symbiotic dinoflagellates (SD) by the corals in response to thermal stress has been described as coral bleaching. A stress damages the photosynthetic apparatus of SD and their thylakoid membranes, and symbiotic alga cells disappear via exocytosis and/or apoptosis resulting in a disturbance of host cell membranes. Lipids play an essential role in coral metabolism and constitute the basis of membranes. At present, studies of coral bleaching typically deal with long-term lipid indices, such as total lipids and fatty acids (FM), while short-term dynamics of lipidome (full range of all lipid molecular species) has not been considered in the context of bleaching mechanism. To evaluate the lipidomic approach to characterizing coral bleaching, the soft coral Sinularia sp. was exposed to a short-term experimental thermal stress (33 degrees C). The dynamics of chlorophyll level, the composition of total FAs, the amounts of total lipids and major lipid classes (such as wax esters (WE), triacylglycerols (TAG), and monoalkyldiacylglycerols (MADAG), glycolipids, ethanolamine-, choline-, serine-, inositolglycerophospholipids (PE, PC, PS, PI), and ceramide aminoethylphosphonate (CAEP)) and the variations in the content of lipid molecular species were studied during a 36-h heat exposure. > 160 molecular species were identified by tandem high-resolution mass spectrometry. Under the short-term stress, a considerable decline in the levels of chlorophylls, glycolipids, and FA markers of SD was detected, whereas the variations in the content of total lipids, total phospholipids, and major FAs were not significant. The heat exposure immediately disturbed the daily cycle of the composition of glycolipid molecular species. Among the storage lipids, the content of TAG and MADAG decreased, whereas the content of polyunsaturated WE molecular species increased under the stress. The following effects were recorded for the structural lipids: a depletion in the PE, PC, and PI amounts, a conversion of PE into their lyso and oxidized derivatives, an appearance of diacyl form of PE, and a disturbance of transfer of some polyunsaturated FAs from SD to the host. The content of PS was relatively stable. Probably, PE molecular species are a primary target of thermal stress in the Sinularia host. The most dramatical changes in the Sinularia lipidome were detected between 24 h and 36 h of the exposure. A lipidomic approach is important for studies of dynamics of the coral symbiotic association and a resistance of different coral taxa during short-term bleaching events.
机译：珊瑚对热胁迫的反应完全或部分丧失了共生的鞭毛藻（SD），这被称为珊瑚漂白。应激会破坏SD的光合装置及其类囊体膜，共生藻类细胞会通过胞吐作用和/或凋亡而消失，从而导致宿主细胞膜的紊乱。脂质在珊瑚的新陈代谢中起着至关重要的作用，并构成了膜的基础。目前，对珊瑚漂白的研究通常涉及长期脂质指数，例如总脂质和脂肪酸（FM），而在此背景下尚未考虑脂质组的短期动力学（所有脂质分子种类的全部范围）漂白机理。为了评估表征珊瑚褪色的脂质组学方法，软珊瑚Sinularia sp。暴露于短期实验热应力（33摄氏度）。叶绿素水平，总脂肪酸组成，总脂质含量和主要脂质类别（例如蜡酯（WE），三酰基甘油（TAG）和单烷基二酰基甘油（MADAG），糖脂，乙醇胺，胆碱，丝氨酸）的动力学-在36小时的热暴露过程中研究了肌醇甘油磷脂（PE，PC，PS，PI）和神经酰胺氨基乙基膦酸酯（CAEP）以及脂质分子种类含量的变化。通过串联高分辨率质谱法鉴定出> 160个分子种类。在短期胁迫下，检测到SD的叶绿素，糖脂和FA标记水平显着下降，而总脂质，总磷脂和主要FA的含量变化不明显。热暴露立即干扰了糖脂分子种类的组成的日常循环。在贮藏脂质中，TAG和MADAG的含量降低，而多不饱和WE分子种类的含量在胁迫下增加。记录了以下结构脂质的作用：PE，PC和PI量的减少，PE转化为其溶血和氧化衍生物，PE的二酰基形式出现以及某些多不饱和FA转移的干扰从SD到主机。 PS的含量相对稳定。 PE分子物种可能是Sinularia宿主中热应激的主要目标。在暴露的24 h至36 h之间检测到了Sinularia脂质组中最显着的变化。脂质组学方法对于研究珊瑚共生缔合的动力学以及短期漂白事件中不同珊瑚类群的抗性很重要。

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