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首页> 外文期刊>Journal of Experimental Marine Biology and Ecology >Protein in marine plankton: A comparison of spectrophotometric methods
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Protein in marine plankton: A comparison of spectrophotometric methods

机译：海洋浮游生物中的蛋白质：分光光度法的比较

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摘要

Measuring protein is critical to many investigations in oceanography and marine biology. Here, we compared seven colorimetric protein assays (Rutter, Rutter-SDS, Markwell, BCA, microBCA, Bradford and microBradford) for measuring protein in a mysid (Leptomysis lingvura), in a jellyfish (Pelagia noctiluca), and in three different size fractions of marine plankton (0.7-50, 50-200 and 200-2000 mu m). Significant differences occurred in all of these samples. However, the mBCA method was the most accurate for all samples except the mysid, the Rutter method was the least accurate in all organisms, and the Bradford and microBradford methods consistently under-estimated protein. The time-dependent behavior of the protein signal was most accurately determined if the analysis was carried out rapidly and consistently. In relation to the limit of detection (LOD), the most sensitive method for low protein levels was the microBCA assay (at 7 mu g mL(-1) protein). The most sensitive method at higher levels of protein was the Bradford method (at 472 mu g mL(-1) protein). The BCA method was the most linear and the microBCA method, the most sensitive method for detecting bovine serum albumin. We recommend the latter two methods for measuring protein under our assay conditions.
机译：测量蛋白质对于海洋学和海洋生物学的许多研究至关重要。在这里，我们比较了七个比色蛋白测定法（Rutter，Rutter-SDS，Markwell，BCA，microBCA，Bradford和microBradford），用于测定甲壳（（Leptomysis lingvura），水母（Pelagia noctiluca）和三种不同大小的级分中的蛋白质海洋浮游生物（0.7-50、50-200和200-2000微米）。在所有这些样品中均发生了显着差异。但是，对于所有样品，mBSA方法对所有样品的准确性最高，而罗氏方法在所有生物中的准确性均最低，而Bradford和microBradford方法始终低估了蛋白质。如果快速，一致地进行分析，则可以最准确地确定蛋白质信号的时间依赖性行为。关于检测限（LOD），低蛋白水平最敏感的方法是microBCA分析（7μg mL（-1）蛋白）。蛋白质含量较高时最敏感的方法是Bradford方法（472μg mL（-1）蛋白质）。 BCA方法是最线性的方法，而microBCA方法是检测牛血清白蛋白的最灵敏方法。我们建议在我们的测定条件下使用后两种方法测量蛋白质。

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