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Microencapsulation of microbial cells

机译：微生物细胞的微囊化

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摘要

Microencapsulation involves coating or entrapping of a core material with a polymeric material to generate microspheres in the size range of 1-1000 μm. This versatile technology has been used to encapsulate a wide array of products such as Pharmaceuticals, flavors, volatile oils, plant extracts, enzymes and others. In the recent decades, this technology has also been applied to the area of microbial cell immobilization owing to its numerous advantages over other cell immobilization techniques such as higher cell loading capacity, enhanced cell survival and increased production rate of the desired microbial products. The confinement of microbial cells within a semipermeable polymeric matrix enables the physical isolation of cells from the external environment while maintaining a hospitable internal micro-environment. It has found application in various biotechnological processes such as probiotic encapsulation in food industries, in biotransformation and fermentation processes producing antibiotics, organic acids, enzymes, and alcohols as well as environmental decontamination such as waste water treatment. The judicious selection of materials and methods for the production of microspheres is critical for ensuring minimum damage to the viability of the encapsulated microbial cells. The conventional methods used for microencapsulation of microbial cells are reviewed along with the recent advances in the respective methods. The effect of microencapsulation on the microbial cells, the stability of the microspheres as well as the techniques for enumeration of the encapsulated cells are also discussed, followed by a summary of recent applications of microencapsulation in different biotechnological processes.
机译：微囊化涉及用聚合材料涂覆或包裹核材料，以产生尺寸范围为1-1000μm的微球。这种通用技术已被用于封装多种产品，例如药品，调味剂，挥发油，植物提取物，酶等。在最近几十年中，由于该技术相对于其他细胞固定技术具有众多优势，例如更高的细胞负载能力，增强的细胞存活率和所需微生物产品的生产率提高，因此该技术也已应用于微生物细胞固定领域。将微生物细胞限制在半透性聚合物基质中，可以使细胞与外部环境物理隔离，同时保持好客的内部微环境。它已发现可用于各种生物技术过程，例如食品工业中的益生菌封装，生产抗生素，有机酸，酶和醇的生物转化和发酵过程，以及废水处理等环境净化过程。明智地选择用于生产微球的材料和方法，对于确保对包封的微生物细胞生存能力的损害最小化至关重要。综述了用于微囊化微生物细胞的常规方法以及相应方法的最新进展。还讨论了微囊化对微生物细胞的影响，微球的稳定性以及囊化细胞的计数技术，随后总结了微囊化在不同生物技术过程中的最新应用。

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  • 来源
    《Journal of food engineering》 |2013年第2期|369-381|共13页
  • 作者

    Sweta Rathore; Parind Mahendrakumar Desai; Celine Valeria Liew; Lai Wah Chan; Paul Wan Sia Heng;

  • 作者单位

    GEA-NUS Pharmaceutical Processing Research Laboratory, Department of Pharmacy, National University of Singapore, 18 Science Drive 4, Singapore 117543, Singapore;

    GEA-NUS Pharmaceutical Processing Research Laboratory, Department of Pharmacy, National University of Singapore, 18 Science Drive 4, Singapore 117543, Singapore;

    GEA-NUS Pharmaceutical Processing Research Laboratory, Department of Pharmacy, National University of Singapore, 18 Science Drive 4, Singapore 117543, Singapore;

    GEA-NUS Pharmaceutical Processing Research Laboratory, Department of Pharmacy, National University of Singapore, 18 Science Drive 4, Singapore 117543, Singapore;

    GEA-NUS Pharmaceutical Processing Research Laboratory, Department of Pharmacy, National University of Singapore, 18 Science Drive 4, Singapore 117543, Singapore;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    immobilization; microencapsulation; microbial cells; stability;

    机译：固定微囊化微生物细胞稳定性;

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