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首页> 外文期刊>Journal of food protection >Efficacy of Sanitizing Agents against Listeria monocytogenes Biofilms on High-Density Polyethylene Cutting Board Surfaces
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Efficacy of Sanitizing Agents against Listeria monocytogenes Biofilms on High-Density Polyethylene Cutting Board Surfaces

机译：消毒剂对高密度聚乙烯切菜板表面单核细胞增生李斯特菌生物膜的功效

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摘要

This study compared the effectiveness of 10 commercially available sanitizers against Listeria monocytogenes biofilms on high-density polyethylene cutting boards. Smooth and rough surface high-density polyethylene coupons (2 by 5 cm) were inoculated (approximately 6 log CFU/cm~2) with a five-strain composite of L monocytogenes in ham homogenate and incubated at 24℃ and ≥90% relative humidity for up to 21 days. The coupons were subjected to repeated 24-h cycles simulating use and cleaning in the home. Each day, 0.3 ml of a 10-fold-diluted tryptic soy broth containing 0.6% yeast extract was added to each coupon (simulating exposure to nutrients during food preparation), and 8 h later each coupon was rinsed with sterile distilled water. Coupons were subjected to sanitizer treatments on days 0, 0.25, 7, 14, and 21. Eight quaternary ammonium compound (QAC)-based sanitizers, one of lactic acid-based sanitizer, and one sodium hypochlorite-based sanitizer were applied to individual coupons according to the manufacturers' instructions. Coupons were analyzed for L. monocytogenes (PALCAM agar) and total bacteria (tryptic soy agar with 0.6% yeast extract). At 0 and 0.25 days, nine of the sanitizers (all except for QAC-based sanitizer 10) had reduced L. monocytogenes to <0.60 log CFU/cm~2. For ≥7-day-old biofilms, the lactic acid-based sanitizer (pH 3.03) was the most effective, and the QAC-based sanitizers were more effective when at pH 10.42 to 11.46 than at pH 6.24 to 8.70. Sanitizer efficacies were greater (P < 0.05) against younger (7 days) than older (21 days) biofilms on smooth surfaces. For 7- and 14-day-old biofilms, sanitizer efficacies were higher (P < 0.05) on smooth than on rough surfaces.
机译：这项研究比较了10种市售消毒剂对高密度聚乙烯切菜板上的单核细胞增生李斯特菌生物膜的有效性。在火腿匀浆中用五株单核细胞增生李斯特氏菌复合物接种光滑和粗糙的高密度聚乙烯试样（2 x 5 cm）（约6 log CFU / cm〜2），并在24℃和≥90％相对湿度下孵育长达21天。试样被重复24小时循环以模拟在家里的使用和清洁。每天，将0.3 ml的含有0.6％酵母提取物的10倍稀释的胰蛋白酶大豆肉汤添加到每个样片中（模拟食品制备过程中暴露于营养中），然后8小时后，用无菌蒸馏水冲洗每个样片。对优惠券在第0、0.25、7、14和21天进行消毒处理。将八种基于季铵化合物（QAC）的消毒剂，一种基于乳酸的消毒剂和一种基于次氯酸钠的消毒剂应用于各个优惠券。根据制造商的说明。分析优惠券中的单核细胞增生李斯特氏菌（PALCAM琼脂）和总细菌（胰蛋白酶大豆琼脂含0.6％酵母提取物）。在第0天和0.25天，有9个消毒剂（除基于QAC的消毒剂10以外的所有消毒剂）已将单核细胞增生李斯特氏菌减少至<0.60 log CFU / cm〜2。对于≥7天的生物膜，基于乳酸的消毒剂（pH 3.03）最有效，而当pH为10.42至11.46时，基于QAC的消毒剂比在pH 6.24至8.70时更有效。在光滑表面上，针对年轻（7天）的生物膜的消毒效果要比针对老年人（21天）的生物膜更大（P <0.05）。对于7天和14天大的生物膜，在光滑表面上的消毒效果要比在粗糙表面上更高（P <0.05）。

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  • 来源
    《Journal of food protection》 |2009年第5期|990-998|共9页
  • 作者

    HUA YANG; PATRICIA A. KENDALL; LYDIA C. MEDEIROS; JOHN N. SOFOS;

  • 作者单位

    Center for Meat Safety & Quality and Food Safety Cluster, Department of Animal Sciences, Colorado State University, Fort Collins, Colorado 80523;

    Department of Food Science and Human Nutrition, Colorado State University, Fort Collins, Colorado 80523;

    Department of Human Nutrition, Ohio State University, Columbus, Ohio 43210, USA;

    Center for Meat Safety & Quality and Food Safety Cluster, Department of Animal Sciences, Colorado State University, Fort Collins, Colorado 80523;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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