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首页> 外文期刊>Journal of food protection >Seasonal prevalence of Shiga toxin-producing Escherichia coli, including O157:H7 and non-O157 serotypes, and Salmonella in commercial beef processing plants.
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Seasonal prevalence of Shiga toxin-producing Escherichia coli, including O157:H7 and non-O157 serotypes, and Salmonella in commercial beef processing plants.

机译：产志贺毒素的大肠杆菌在商业牛肉加工厂的季节性流行，包括O157：H7和非O157血清型以及沙门氏菌。

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The seasonal prevalence of Escherichia coli O157:H7, Salmonella, non-O157 E. coli (STEC), and stx-harboring cells was monitored at three Midwestern fed-beef processing plants. Overall, E. coli O157:H7 was recovered from 5.9% of fecal samples, 60.6% of hide samples, and 26.7% of carcasses sampled before the preevisceration wash. This pathogen also was recovered from 1.2% (15 of 1,232) of carcasses sampled at chilling (postintervention) at approximate levels of <3.0 cells per 100 cm2. In one case, the E. coli O157:H7 concentration dropped from ca. 1,100 cells per 320 cm2 at the preevisceration stage to a level that was undetectable on ca. 2,500 cm2 at the postintervention stage. The prevalence of E. coli O157:H7 in feces peaked in the summer, whereas its prevalence on hide was high from the spring through the fall. Overall, Salmonella was recovered from 4.4, 71.0, and 12.7% of fecal, hide, and preevisceration carcass samples, respectively. Salmonella was recovered from one postintervention carcass (of 1,016 sampled). Salmonella prevalence peaked in feces in the summer and was highest on hide and preevisceration carcasses in the summer and the fall. Non-O157 STEC prevalence also appeared to vary by season, but the efficiency in the recovery of isolates from stx-positive samples ranged from 37.5 to 83.8% and could have influenced these results. Cells harboring stx genes were detected by PCR in 34.3, 92.0, 96.6, and 16.2% of fecal, hide, preevisceration carcass, and postintervention carcass samples, respectively. The approximate level of non-O157 STEC and stx-harboring cells on postintervention carcasses was > or = 3.0 cells per 100 cm2 for only 8 of 199 carcasses (4.0%). Overall, the prevalence of E. coli O157:H7, Salmonella, and non-O157 STEC varied by season, was higher on hides than in feces, and decreased dramatically, along with pathogen levels, during processing and during the application of antimicrobial interventions. These results demonstrate the effectiveness of the current interventions used by the industry and highlight the significance of hides as a major source of pathogens on beef carcasses.
机译：在三个中西部的饲喂牛肉加工厂中监测了O157：H7大肠杆菌，沙门氏菌，非O157大肠杆菌（STEC）和stx感染细胞的季节性流行情况。总体而言，在除酸洗之前，从5.9％的粪便样本，60.6％的皮革样本和26.7％的尸体样本中回收了O157：H7大肠杆菌。该病原体还可以从冷藏（干预后）1.2％（1,232个中的15个）屠体中以低于每100 cm2少于3.0个细胞的水平回收。在一种情况下，大肠杆菌O157：H7的浓度从大约在除斑阶段，每320 cm2 1,100个单元达到无法检测到的水平。干预后阶段的面积为2500平方厘米。粪便中大肠杆菌O157：H7的流行率在夏季达到高峰，而从春季到秋季，皮革中的流行率很高。总体而言，沙门氏菌分别从粪便，生皮和内脏car体样品中回收4.4、71.0和12.7％。从一个干预后的car体中（从1,016个样本中）回收沙门氏菌。沙门氏菌的流行率在夏季的粪便中达到最高，在夏季和秋季的皮革和去内脏屠体中最高。非O157 STEC的患病率也随季节而变化，但是从stx阳性样品中分离出的分离物的回收效率在37.5％至83.8％之间，可能影响了这些结果。通过PCR检测分别在粪便，生皮，去内脏pre体和干预后car体样品中的34.3％，92.0％，96.6％和16.2％携带stx基因的细胞。干预后屠体上非O157 STEC和stx吞噬细胞的大概水平是每100 cm2>或= 3.0个细胞，仅199个屠体中有8个（4.0％）。总体而言，大肠杆菌O157：H7，沙门氏菌和非O157 STEC的患病率随季节而变化，在生皮中比在粪便中更高，并且在加工过程和应用抗菌干预过程中，与病原体水平一起急剧下降。这些结果证明了该行业当前采取的干预措施的有效性，并突出了生皮作为牛肉car体病原体主要来源的重要性。

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