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首页> 外文期刊>Journal of Food Science >The Phenolic Compounds, Metabolites, and Antioxidant Activity of Propolis Extracted, by Ultrasound-Assisted Method
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The Phenolic Compounds, Metabolites, and Antioxidant Activity of Propolis Extracted, by Ultrasound-Assisted Method

机译：通过超声辅助方法提取蜂胶化合物，代谢物和丙醇的抗氧化活性

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摘要

The influences of ultrasound-assisted, pharmacopeia, and supercritical fluid extraction methods on bioactive compounds and biological activities of propolis were evaluated. Results showed that propolis extracted by ultrasound-assisted method contained more phenolic compounds, and showed the highest total phenolic content (245.84 ± 6.41 mg GAE/g DW), total flavonoids content (198.82 ± 5.74 mg RE/g DW), and stronger in vitro antioxidant activity (DPPH-: 1.03 ± 0.04 mmol Trolox/g DW, ABTS+-: 2.19 ± 0.05 mmol Trolox/g DW, and FRAP: 1.48 ± 0.12 mmol FeSCVg DW) than those of pharmacopoeia and supercritical fluid methods. A total of 36 phenolic compounds were identified in propolis. Among them, quercetin, quercetin-3-methyl-ether, kaempferol, isorhamnetin, luteolin-methyl-ether, and quercetin-7-methyl-ether could only be found in ultrasound-assisted and pharmacopoeia methods. Moreover, the phenolic compounds had the similar metabolic pathways in rats and were mainly metabolized by sulfation and glucuronidation pathways. Additionally, ultrasonic-treated propolis have good in vivo antioxidant activity and could repair D-galactose-induced oxidative damage in rats. Therefore, ultrasound-assisted method could replace pharmacopeia method to be considered as bioactive compounds extraction from propolis, taking into consideration of yield, short extraction time, and high antioxidant activity.
机译：评价超声辅助，药物岩和超临界流体提取方法对蜂胶生物活性化合物和生物活性的影响。结果表明，通过超声辅助方法提取的蜂胶含有更多的酚类化合物，并显示出最高的总酚含量（245.84±6.41mg，总黄酮含量（198.82±5.74mg RE / G DW），更强大体外抗氧化活性（DPPH - ：1.03±0.04mmol Trolox / G DW，ABTS + - ：2.19±0.05 mmol TroLox / G DW，FRAP：1.48±0.12mmol FESCVG DW）比药典和超临界流体方法。在蜂胶中鉴定了总共36种酚类化合物。其中，槲皮素，槲皮素-3-甲基 - 醚，Kaempferol，isorhamnetin，甲状腺素 - 甲基 - 醚和槲皮素-7-甲基 - 醚只能在超声辅助和药典方法中发现。此外，酚类化合物在大鼠中具有相似的代谢途径，主要通过硫化和葡糖醛途径代谢。另外，超声处理的蜂胶具有良好的体内抗氧化活性，并且可以修复D-半乳糖诱导的大鼠氧化损伤。因此，考虑到产率，短的提取时间和高抗氧化活性，超声辅助方法可以取代药典方法被认为是从蜂胶中提取的生物活性化合物。

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  • 来源
    《Journal of Food Science》 |2019年第12期|3850-3865|共16页
  • 作者

    Yuan Yuan; Shilian Zheng; Linhui Zeng; Zeyuan Deng; Bing Zhang; Hongyan Li;

  • 作者单位

    State Key Laboratory of Food Science and Technology Univ. of Nanchang Nanchang 330047 Jiangxi China;

    State Key Laboratory of Food Science and Technology Univ. of Nanchang Nanchang 330047 Jiangxi China;

    State Key Laboratory of Food Science and Technology Univ. of Nanchang Nanchang 330047 Jiangxi China;

    State Key Laboratory of Food Science and Technology Univ. of Nanchang Nanchang 330047 Jiangxi China;

    State Key Laboratory of Food Science and Technology Univ. of Nanchang Nanchang 330047 Jiangxi China;

    State Key Laboratory of Food Science and Technology Univ. of Nanchang Nanchang 330047 Jiangxi China;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    antioxidant activity; metabolism; phenolic compounds; propolis; ultrasound-assisted extraction;

    机译：抗氧化活性;代谢;酚类化合物;蜂胶;超声波辅助提取;

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