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首页> 外文期刊>Journal of Food Science >Optimization of ultrasonic-assisted extraction conditions for bioactive components from coffee leaves using the Taguchi design and response surface methodology
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Optimization of ultrasonic-assisted extraction conditions for bioactive components from coffee leaves using the Taguchi design and response surface methodology

机译:使用TAGUCHI设计和响应表面方法从咖啡叶中获得超声辅助提取条件的超声波辅助提取条件

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摘要

Coffee leaves contain various bioactive compounds that are beneficial for human health. However, there are very limited researches related to the extraction of the bioactive phytochemicals from coffee leaves. In the present study, the extraction conditions for bioactive components from coffee leaves were optimized using Taguchi design and response surface methodology (RSM). Taguchi design was used to screen significant factors that affected the yield of phytochemicals including trigonelline, caffeine, chlorogenic acids, mangiferin, and rutin, total phenolic content (TPC) and antioxidant activity. Sequentially, a Box-Behnken design (BBD) was used to optimize the extraction conditions. Three factors including Liquid-to-solid (L:S) ratio, ethanol concentration, and extraction temperature that significantly affected most of the phytochemical yields and antioxidant activity were selected from the six variables using Taguchi design. The optimal extraction conditions obtained from RSM were 30.3:1 L:S ratio, 54.5% ethanol, and 80 °C when simultaneously considered four responses, including TPC, the yields of mangiferin and 5-CQA and DPPH scavenging capacity. Under the optimal conditions, the experimental results for the above four responses were 62.1 mg gallic acid/g, 4.1 mg/g, 11.4 mg/g, and 356.9 μmol Trolox/g, respectively, which were close to the predicted values. About 97% of phytochemicals can be extracted in the first two times of extraction. In conclusion, the combination of Taguchi design and response surface methodology can be successfully used to screen and optimize the significant factors that affected the bioactive components extracted from coffee leaves.
机译:咖啡叶含有各种对人体健康有益的生物活性化合物。然而,与来自咖啡叶的生物活性植物化学物质的提取有很大的研究。在本研究中,使用Taguchi设计和响应表面方法(RSM)优化来自咖啡叶的生物活性成分的提取条件。 Taguchi设计用于筛选影响植物化学物质产量的重要因素,包括三角形,咖啡因,绿原酸,Mangiferin和芦丁,总酚类含量(TPC)和抗氧化活性。顺序地,用于优化提取条件的Box-Behnken设计(BBD)。三种因素包括液体 - 固体(L:S)比,乙醇浓度和显着影响大部分植物化学产率和抗氧化活性的萃取温度,选自Taguchi设计的六个变量。当同时考虑四种反应时,从RSM获得的最佳提取条件为30.3:1L:S比,54.5%乙醇和80℃,包括TPC,Mangiferin和5-CQA和DPPH清除能力的产量。在最佳条件下,上述四种反应的实验结果分别为62.1mg,4.1mg / g,11.4mg / g,4.1mg / g,11.4mg / g和356.9μmolrotox / g,其接近预测值。在提取的前两次,可以提取约97%的植物化学物质。总之,Taguchi设计和响应表面方法的组合可以成功地用于筛选并优化影响从咖啡叶中提取的生物活性成分的重要因素。

著录项

  • 来源
    《Journal of Food Science》 |2020年第6期|1742-1751|共10页
  • 作者单位

    School of Food and Biological Engineering Jiangsu University 301 Xuefu Road Jingkou District Zhenjiang Jiangsu 212013 PR. China Institute of Food Physical Processing Jiangsu University 301 Xuefu Road Jingkou District Zhenjiang Jiangsu 212013 PR. China International Research Center for Food Nutrition and Safety Jiangsu University Zhenjiang 212013 China;

    School of Food and Biological Engineering Jiangsu University 301 Xuefu Road Jingkou District Zhenjiang Jiangsu 212013 PR. China;

    School of Food and Biological Engineering Jiangsu University 301 Xuefu Road Jingkou District Zhenjiang Jiangsu 212013 PR. China;

    School of Food and Biological Engineering Jiangsu University 301 Xuefu Road Jingkou District Zhenjiang Jiangsu 212013 PR. China;

    School of Food and Biological Engineering Jiangsu University 301 Xuefu Road Jingkou District Zhenjiang Jiangsu 212013 PR. China Institute of Food Physical Processing Jiangsu University 301 Xuefu Road Jingkou District Zhenjiang Jiangsu 212013 PR. China;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Coffee leaves; ultrasonic-assisted extraction; Box-Behnken design; Taguchi design; antioxidant;

    机译:咖啡叶;超声波辅助提取;Box-Behnken设计;Taguchi设计;抗氧化剂;

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