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Optimization of extraction conditions for pollock skin gelatin

机译：鳕鱼皮明胶提取条件的优化

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Seven potential variables, pretreatment temperature, pretreatment time, concentration of OH-, concentration of H+, extraction temperature, extraction time, and skin/water ratio, were investigated by a 2(7-3) fractional factorial design to identify critical control factors for pollock skin gelatin extraction, and 3 responses, yield, gel strength, and viscosity, were evaluated. The results suggest that 4 variables, pretreatment temperature, concentration of OH-, concentration of H+, and extraction temperature, have significant effects on gelatin extraction, and these key factors were then selected for the subsequent optimization using response surface methodology with a 4-factor, 5-level central composite rotatable design. It suggests that a concentration of OH- at 0.25 mol/L, a concentration of H+ at 0.09 mol/L, a pretreatment temperature at 2 degreesC, and an extraction temperature at 50 degreesC, will give the highest values for the 3 responses. The predicted responses for these extraction conditions are that the yield will be 18%, gel strength will be 460 g, and viscosity will be 6.2 cP.
机译：通过2（7-3）分数阶乘设计研究了七个潜在变量，即预处理温度，预处理时间，OH-浓度，H +浓度，提取温度，提取时间和皮肤/水比，以确定关键的控制因素。评估了鳕鱼皮明胶的提取率，并评估了3种响应，产量，凝胶强度和粘度。结果表明，预处理温度，OH-浓度，H +浓度和提取温度这4个变量对明胶的提取有显着影响，然后使用4因子响应面方法选择了这些关键因素用于随后的优化，5级中央复合旋转设计。这表明在0.25 mol / L的OH-浓度，在0.09 mol / L的H +浓度，2摄氏度的预处理温度和50摄氏度的萃取温度将为这3种响应提供最高的值。对于这些提取条件的预测响应是收率将达到18％，凝胶强度将达到460 g，粘度将达到6.2 cP。

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