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首页> 外文期刊>Journal of Genetics >Genetic variability and relationship between MT-1 elephant grass and closely related cultivars assessed by SRAP markers
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Genetic variability and relationship between MT-1 elephant grass and closely related cultivars assessed by SRAP markers

机译：用SRAP标记评估MT-1象草与紧密相关品种的遗传变异及相关性

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摘要

Genetic variability and relationships among elephant grass cultivars were estimated by the SRAP (sequence-related amplified polymorphism) assay. A total of 60 individuals collected from five cultivars in China were analysed. Sixty-two selected primer combinations generated 1395 bands, with an average of 22.5 per primer combination. The average value of percentage of polymorphic bands (PPB) was 72.8% at species level. The PPB was from 15.2% to 75%, with an average of 39.6% at cultivar level. H POP , within-cultivar Shannon’s index was 1.738 at cultivar level; at species level, the Shannon’s index (H SP ) was 3.880. An assessment of diversity between cultivars [(H SP − H POP )/H SP ] indicated that most of the diversity (55.2%) was detected among cultivars, and only 44.8% was within cultivars in total genetic variation. According to UPGMA dendrogram, the five cultivars were clustered into three main groups. One group included MT-1 and Mott with a bootstrap support of 100%, another consisted of Huanan and N51 with a bootstrap support of 81%, and last one was only Guimu-1. The results indicate that the MT-1 and Mott have a closest genetic relationship; Huanan and N51 possess a relatively close relationship, and Guimu-1 is the most distinct from the other four cultivars. Keywords genetic diversity - cluster analysis - DNA markers - Shannon information index - Pennisetum purpureum
机译：通过SRAP（序列相关的扩增多态性）分析来估计象草品种之间的遗传变异性和相关性。分析了来自中国五个品种的60个个体。选择的62个引物组合产生了1395条条带，平均每个引物组合为22.5条。在物种水平上，多态性带（PPB）百分比的平均值为72.8％。 PPB从15.2％到75％，在品种水平上平均为39.6％。 H POP ，品种内的Shannon指数为1.738；在物种水平上，香农指数（H SP ）为3.880。品种间[（H SP -H POP ）/ H SP ]之间的多样性评估表明，大部分多样性（55.2％）是在品种中检测到，只有44.8％位于总遗传变异中。根据UPGMA树状图，这五个品种被分为三个主要类别。一组包括MT-1和Mott，具有100％的自举支持，另一组包括华南和N51，具有81％的自举支持，最后一组仅是Guimu-1。结果表明MT-1和Mott具有最密切的遗传关系。华南与N51的关系相对较近，而贵木1号与其他4个品种最不相同。关键词遗传多样性聚类分析DNA标记Shannon信息指数紫狼尾草

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