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首页> 外文期刊>Journal of Genetics >Simple sequence repeat marker development and genetic mapping in quinoa (Chenopodium quinoa Willd.)
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Simple sequence repeat marker development and genetic mapping in quinoa (Chenopodium quinoa Willd.)

机译：藜麦（Chenopodium quinoa Willd。）中简单的序列重复标记开发和遗传定位

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Quinoa is a regionally important grain crop in the Andean region of South America. Recently quinoa has gained international attention for its high nutritional value and tolerances of extreme abiotic stresses. DNA markers and linkage maps are important tools for germplasm conservation and crop improvement programmes. Here we report the development of 216 new polymorphic SSR (simple sequence repeats) markers from libraries enriched for GA, CAA and AAT repeats, as well as 6 SSR markers developed from bacterial artificial chromosome-end sequences (BES-SSRs). Heterozygosity (H) values of the SSR markers ranges from 0.12 to 0.90, with an average value of 0.57. A linkage map was constructed for a newly developed recombinant inbred lines (RIL) population using these SSR markers. Additional markers, including amplified fragment length polymorphisms (AFLPs), two 11S seed storage protein loci, and the nucleolar organizing region (NOR), were also placed on the linkage map. The linkage map presented here is the first SSR-based map in quinoa and contains 275 markers, including 200 SSR. The map consists of 38 linkage groups (LGs) covering 913 cM. Segregation distortion was observed in the mapping population for several marker loci, indicating possible chromosomal regions associated with selection or gametophytic lethality. As this map is based primarily on simple and easily-transferable SSR markers, it will be particularly valuable for research in laboratories in Andean regions of South America.
机译：藜麦是南美安第斯地区的一种重要的区域性粮食作物。最近，藜麦因其高营养价值和对极端非生物胁迫的耐受性而受到国际关注。 DNA标记和连锁图谱是种质保护和作物改良计划的重要工具。在这里，我们报道了从富含GA，CAA和AAT重复序列的文库中开发了216个新的多态性SSR（简单序列重复）标记，以及从细菌人工染色体末端序列（BES-SSR）开发的6个SSR标记。 SSR标记的杂合度（H）值介于0.12至0.90之间，平均值为0.57。使用这些SSR标记为新开发的重组自交系（RIL）群体构建了连锁图。其他标记，包括扩增的片段长度多态性（AFLP），两个11S种子贮藏蛋白基因座和核仁组织区（NOR），也放置在连锁图上。此处显示的连锁图是奎奴亚藜中第一个基于SSR的图，包含275个标记，包括200个SSR。该地图由38个链接组（LG）组成，覆盖913 cM。在作图群体中观察到几个标记位点的分离畸变，表明可能存在与选择或配子体致死性相关的染色体区域。由于该地图主要基于简单且易于转移的SSR标记，因此对于南美安第斯地区的实验室研究尤其有价值。

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