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首页> 外文期刊>Journal of Hazardous Materials >Co-expression of multiple heavy metal transporters changes the translocation, accumulation, and potential oxidative stress of Cd and Zn in rice (Oryza sativa)
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Co-expression of multiple heavy metal transporters changes the translocation, accumulation, and potential oxidative stress of Cd and Zn in rice (Oryza sativa)

机译:多种重金属转运体的共表达改变了水稻中Cd和Zn的转运,积累和潜在的氧化胁迫

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摘要

The OsHMA2, OsLCT1 and OsZIP3 transporters were all involved in zinc (Zn) and cadmium (Cd) transport. So far, only a few researches studied on the co-regulation effect of three transporters related to Zn and Cd transport. The present study showed that rice co-expressing OsLCT1-OsHMA2-OsZIP3 (LHZ) had longer roots and shoots than wild-type (WT) rice after Zn and Cd treatments. The chlorophyll content was significantly higher, and the proline, malondialdehyde and H2O2 contents were significantly lower in co-transgenic lines than in WT under Cd and Zn stress. LHZ in the seedlings of transgenic rice decreased the root-to-shoot translocation of Cd after Cd and Zn treatments. At the filling stage, LHZ line reduced Cd accumulation in grain after Cd treatment. Moreover, LHZ line increased the translocation of Zn to grain and reduced the accumulation of Cd after Zn treatment. These results suggested that LHZ co-expression could effectively decrease the translocation and accumulation of Cd to grains, alleviated the oxidative stress of Cd and Zn, and finally enhanced the quality and safety of rice grains.
机译:OsHMA2,OsLCT1和OsZIP3转运蛋白均参与锌(Zn)和镉(Cd)转运。迄今为止,只有很少的研究研究了与锌和镉的运输有关的三种运输子的共调节作用。本研究表明,共表达OsLCT1-OsHMA2-OsZIP3(LHZ)的稻根和芽比野生型(WT)稻经过锌和镉处理后更长。在镉和锌胁迫下,共转基因品系中的叶绿素含量显着更高,脯氨酸,丙二醛和H2O2含量显着低于野生型。转Cd和Zn处理后,转基因水稻幼苗中的LHZ降低了Cd的根茎移位。在灌装阶段,LHZ系降低了Cd处理后谷物中Cd的积累。此外,LHZ系增加了Zn处理后Zn向籽粒的转运,并减少了Cd的积累。这些结果表明,LHZ共表达可有效减少Cd向籽粒的转运和积累,减轻Cd和Zn的氧化应激,最终提高稻谷的品质和安全性。

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