...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Hazardous Materials >The role of ATF6 in Cr(Ⅵ)-induced apoptosis in DF-1 cells
【24h】

The role of ATF6 in Cr(Ⅵ)-induced apoptosis in DF-1 cells

机译：ATF6在Cr（Ⅵ）中的作用 - 诱导DF-1细胞的细胞凋亡

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Hexavalent chromium (Cr(VI)) is a common heavy metal pollutant in environment and has been proved possessing the cytotoxicity. In this study, we aimed to investigate the role of activating transcription factor 6 (ATF-6) in apoptosis of chicken embryo fibroblasts cell line (DF-1) induced by Cr(VI). Firstly, DF-1 cells were exposed to Cr(VI) to establish the cytotoxicity model, then the cell apoptosis and ATF-6 protein level were analyzed. By silencing ATF-6 gene, changes of the apoptosis rate and apoptotic proteins were examined. To further explore the regulatory mechanism of ATF-6, endoplasmic reticulum (ER) stress, mitochondrial function, reactive oxygen species (ROS) level, as well as the related pathway were evaluated. Results showed that Cr(VI) can result in DF-1 cell apoptosis, along with mitochondrial membrane potential (MMP) reducing and ER stress. Meanwhile, ATF-6 silencing lowered the apoptosis rate and ER stress level, showing with the decrease of XBP-1, PERK, GRP78, Caspase-12, Cleaved Caspase-3 and the increase of Bcl-2. Further analysis found that ATF-6 silencing downregulated ROS and caused MMP loss, suggesting that ATF-6 silencing inhibited Cr(VI)-induced mitochondrial damage. In conclusion, this study indicate that ATF-6 plays an important regulatory role in Cr(VI)-induced DF-1 cell apoptosis through the ER stress and mitochondrial pathway.
机译：六价铬（Cr（VI））是环境中常见的重金属污染物，并已被证明具有细胞毒性。在这项研究中，我们旨在探讨激活转录因子6（ATF-6）在Cr（VI）诱导的鸡胚胚胎细胞系（DF-1）凋亡中的作用。首先，将DF-1细胞暴露于Cr（VI）以建立细胞毒性模型，然后分析细胞凋亡和ATF-6蛋白质水平。通过沉默ATF-6基因，检查凋亡率和凋亡蛋白的变化。为了进一步探索ATF-6，内质网（ER）应激，线粒体功能，反应性氧物质（ROS）水平以及相关途径的调节机制。结果表明，Cr（VI）可以导致DF-1细胞凋亡，以及线粒体膜电位（MMP）还原和ER应力。同时，ATF-6沉默降低了凋亡率和ER应力水平，显示XBP-1，Perk，GRP78，Caspase-12，切割的Caspase-3和Bcl-2的增加。进一步的分析发现，ATF-6沉默下调的ROS并导致MMP损失，表明ATF-6沉默抑制CR（VI）诱导的线粒体损伤。总之，该研究表明，ATF-6在Cr（VI）中发挥着重要的调节作用，诱导了通过ER应激和线粒体途径的DF-1细胞凋亡。

著录项

  • 来源
    《Journal of Hazardous Materials》 |2021年第15期|124607.1-124607.14|共14页
  • 作者

    Zhang Shuo; Zhao Xiaona; Hao Jiajia; Zhu Yiran; Wang Yue; Wang Lumei; Guo Shuhua; Yi Hui; Liu Yongxia; Liu Jianzhu;

  • 作者单位

    Shandong Agr Univ Coll Vet Med Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Res Ctr Anim Dis Control Engn Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Vet Med Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Vet Med Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Vet Med Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Vet Med Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Vet Med Tai An 271018 Shandong Peoples R China;

    Anim Husb & Vet Serv Ctr Taian City Tai An 271000 Shandong Peoples R China;

    Shandong Agr Univ Res Ctr Anim Dis Control Engn Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Vet Med Tai An 271018 Shandong Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Hexavalent chromium; ATF-6; Apoptosis; ER stress; Mitochondria;

    机译：六价铬;ATF-6;凋亡;ER应激;线粒体;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号