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首页> 外文期刊>Journal of Hazardous Materials >Effects of nitrogen metabolism on growth and aflatoxin biosynthesis in Aspergillus flavus
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Effects of nitrogen metabolism on growth and aflatoxin biosynthesis in Aspergillus flavus

机译：氮代谢对黄曲霉生长和黄曲霉毒素生物合成的影响

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摘要

Aflatoxins (AFs), produced mainly by Aspergillus flavus and Aspergillus parasiticus, are strongly toxic and carcinogenic. Here, we showed that glutamine is the optimal nitrogen source for AF-production in A. flavus grown in Czapek Dox medium. Additionally, 4mM glutamine was the threshold for high production of aflatoxin B-1. However, no significant impact of glutamine synthetase inhibitor was detected for on AF biosynthesis. In contrast, rapamycin could significantly suppress the glutamine inducing effect on AFs production, simultaneously inhibiting the fungal growth and conidiation. To identify the genes and regulatory networks involved in AFs biosynthesis, especially concerning the nitrogen source metabolism pathway and the target of rapamycin (TOR) signaling pathway, we obtained transcriptomes for A. flavus under treatment of three nitrogen sources by RNA-sequencing. We identified 1429 differentially expressed genes. Through GO and KEGG pathway analyses, the relationship between nitrogen metabolism and AFs biosynthesis was revealed, and the effects of TOR inhibitor were confirmed. Additionally, the quantitative real-time PCR results verified the credibility and reliability of the RNA-seq data, and were consistent with the other experimental results. Our research laid the foundation for a primary study on the involvement of the nitrogen regulatory network and TOR signaling pathway in AF biosynthesis. (C) 2016 Elsevier B.V. All rights reserved.
机译：黄曲霉毒素（AFs）主要由黄曲霉和寄生曲霉产生，具有剧毒和致癌性。在这里，我们证明了谷氨酰胺是在Czapek Dox培养基中生长的黄曲霉AF生产的最佳氮源。另外，高产黄曲霉毒素B-1的阈值是4mM谷氨酰胺。但是，没有发现谷氨酰胺合成酶抑制剂对房颤的生物合成有显着影响。相反，雷帕霉素可以显着抑制谷氨酰胺对房颤产生的诱导作用，同时抑制真菌的生长和分生。为了确定参与AFs生物合成的基因和调控网络，特别是涉及氮源代谢途径和雷帕霉素（TOR）信号传导途径的靶标，我们通过RNA测序获得了在三种氮源处理下黄曲霉的转录组。我们鉴定了1429个差异表达基因。通过GO和KEGG途径分析，揭示了氮代谢与AFs生物合成的关系，并证实了TOR抑制剂的作用。此外，实时定量PCR结果验证了RNA-seq数据的可靠性和可靠性，并且与其他实验结果一致。我们的研究为氮调控网络和TOR信号通路参与AF生物合成的基础研究奠定了基础。 （C）2016 Elsevier B.V.保留所有权利。

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  • 来源
    《Journal of Hazardous Materials》 |2017年第ptab期|691-700|共10页
  • 作者

    Wang Bin; Han Xiaoyun; Bai Youhuang; Lin Zhenguo; Qiu Mengguang; Nie Xinyi; Wang Sen; Zhang Feng; Zhuang Zhenhong; Yuan Jun; Wang Shihua;

  • 作者单位

    Fujian Agr & Forestry Univ, Sch Life Sci, Key Lab Pathogen Fungi & Mycotoxins Fujian Prov, Key Lab Biopesticide & Chem Biol,Educ Minist, Fuzhou 350002, Peoples R China;

    Fujian Agr & Forestry Univ, Sch Life Sci, Key Lab Pathogen Fungi & Mycotoxins Fujian Prov, Key Lab Biopesticide & Chem Biol,Educ Minist, Fuzhou 350002, Peoples R China;

    Fujian Agr & Forestry Univ, Sch Life Sci, Key Lab Pathogen Fungi & Mycotoxins Fujian Prov, Key Lab Biopesticide & Chem Biol,Educ Minist, Fuzhou 350002, Peoples R China;

    St Louis Univ, Dept Biol, St Louis, MO 63103 USA;

    Fujian Agr & Forestry Univ, Sch Life Sci, Key Lab Pathogen Fungi & Mycotoxins Fujian Prov, Key Lab Biopesticide & Chem Biol,Educ Minist, Fuzhou 350002, Peoples R China;

    Fujian Agr & Forestry Univ, Sch Life Sci, Key Lab Pathogen Fungi & Mycotoxins Fujian Prov, Key Lab Biopesticide & Chem Biol,Educ Minist, Fuzhou 350002, Peoples R China;

    Fujian Agr & Forestry Univ, Sch Life Sci, Key Lab Pathogen Fungi & Mycotoxins Fujian Prov, Key Lab Biopesticide & Chem Biol,Educ Minist, Fuzhou 350002, Peoples R China;

    Fujian Agr & Forestry Univ, Sch Life Sci, Key Lab Pathogen Fungi & Mycotoxins Fujian Prov, Key Lab Biopesticide & Chem Biol,Educ Minist, Fuzhou 350002, Peoples R China;

    Fujian Agr & Forestry Univ, Sch Life Sci, Key Lab Pathogen Fungi & Mycotoxins Fujian Prov, Key Lab Biopesticide & Chem Biol,Educ Minist, Fuzhou 350002, Peoples R China;

    Fujian Agr & Forestry Univ, Sch Life Sci, Key Lab Pathogen Fungi & Mycotoxins Fujian Prov, Key Lab Biopesticide & Chem Biol,Educ Minist, Fuzhou 350002, Peoples R China;

    Fujian Agr & Forestry Univ, Sch Life Sci, Key Lab Pathogen Fungi & Mycotoxins Fujian Prov, Key Lab Biopesticide & Chem Biol,Educ Minist, Fuzhou 350002, Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Aspergillus flavus; Aflatoxins; Nitrogen sources; Glutamine; RNA-seq;

    机译：黄曲霉;黄曲霉毒素;氮源;谷氨酰胺;RNA-seq;

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