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Plasmid-mediated bioaugmentation for the degradation of chlorpyrifos in soil

机译：质粒介导的生物强化作用降解土壤中毒死degradation

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摘要

To overcome the poor survival and low activity of the bacteria used for bioremediation, a plasmid-mediated bioaugmentation method was investigated, which could result in a persistent capacity for the degradation of chlorpyrifos in soil. The results indicate that the pDOC plasmid could transfer into soil bacteria, including members of the Pseudomonas and Staphylococcus genera. The soil bacteria acquired the ability to degrade chlorpyrifos within 5 days of the transfer of pDOC. The efficiency of the pDOC transfer in the soil, as measured by the chlorpyrifos degradation efficiency and the most probable number (MPN) of chlorpyrifos degraders, was influenced by the soil temperature, moisture level and type. The best performance for the transfer of pDOC was observed under conditions of 30 °C and 60% water-holding capacity (WHC). The results presented in this paper show that the transfer of pDOC can enhance the degradation of chlorpyrifos in various soils, although the degradation efficiency did vary with the soil type. It may be concluded that the introduction of plasmids encoding enzymes that can degrade xenobiotics or donor strains harboring these plasmids is an alternative approach in bioaugmentation.
机译：为了克服用于生物修复的细菌的较差的存活率和较低的活性，研究了一种质粒介导的生物增强方法，该方法可能具有持久的土壤毒死rif降解能力。结果表明，pDOC质粒可以转移到土壤细菌中，包括假单胞菌属和葡萄球菌属。在转移pDOC后的5天内，土壤细菌具有降解毒死rif的能力。通过毒死rif降解效率和毒死rif降解物的最可能数量（MPN）衡量，pDOC在土壤中的转移效率受土壤温度，水分含量和类型的影响。在30°C和60％持水量（WHC）的条件下观察到了pDOC转移的最佳性能。本文提出的结果表明，pDOC的转移可以促进毒死rif在各种土壤中的降解，尽管降解效率随土壤类型而变化。可以得出结论，引入编码可降解具有这些质粒的异种生物或供体菌株的酶的质粒是生物增强中的另一种方法。

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  • 来源
    《Journal of Hazardous Materials》 |2012年第30期|p.178-184|共7页
  • 作者

    Qun Zhang; Baichuan Wang; Zhengya Cao; Yunlong Yu;

  • 作者单位

    Institute of Pesticide and Environmental Toxicology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China,Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Hangzhou 310058, PR China';

    Institute of Pesticide and Environmental Toxicology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China,Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Hangzhou 310058, PR China';

    Institute of Pesticide and Environmental Toxicology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China,Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Hangzhou 310058, PR China';

    Institute of Pesticide and Environmental Toxicology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China,Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Hangzhou 310058, PR China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    plasmid transfer; soil; chlorpyrifos; green fluorescent protein (GFP); bioaugmentation;

    机译：质粒转移;泥;毒死rif;绿色荧光蛋白（GFP）;生物强化;

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