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首页> 外文期刊>Journal of Hazardous Materials >Development of immobilized biophotonic beads consisting of Photobacterium leiognathi for the detection of heavy metals and pesticide
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Development of immobilized biophotonic beads consisting of Photobacterium leiognathi for the detection of heavy metals and pesticide

机译：用于检测重金属和农药的固定化由光子细菌组成的生物光子珠的开发

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摘要

The present communication deals with construction of immobilized robust biophotonic bead using P. leiognathi, a marine luminescent bacterium for their possible application in monitoring of environmental toxicants. Immobilization efficiency of agar, carrageenan and sodium alginate was evaluated separately in terms of luminescence response and was recorded as 30.3,77.4 or 99.5%, respectively. Under optimized storage conditions, the luminescent response of P. leiognathi in the immobilized state was studied over a period of 30 days. These biophotonic beads were further used as a rapid and reliable optical biosensing tool for the detection of heavy metals [Hg(II), As(V) or Cd(II)] and pesticide [2,4-dichlorophenoxyacetic acid (2,4-D)] in water systems. The concentration range for the detection of Hg(II), As(V), Cd(II) and 2,4-D was 2-32 ppm, 4-128 ppm, 16-512 ppm and 100-600 ppm, respectively, while corresponding sensitivity threshold was 2.0 ppm, 4.0 ppm, 16.0 ppm and 100 ppm. A comparison of inhibition constant (K_d) (or EC_(20)) values indicated that the sensitivity thresholds rank as Hg(II) ＞ As(V) ＞ Cd(II) ＞ 2,4-D. Moreover, the time taken for the detection of heavy metals and pesticide was less than 30 min. Using the bioluminescence inhibition method, the concentration of heavy metals and pesticide could be predicted.
机译：本来文涉及利用海洋发光细菌Leiognathi固定化坚固的生物光子珠的构建，以用于可能的环境毒物监测。琼脂，角叉菜胶和藻酸钠的固定效率分别根据发光反应进行评估，分别记录为30.3、77.4或99.5％。在优化的储存条件下，研究了在固定状态下30天内固定状态下的白僵菌的发光反应。这些生物光子珠还被用作快速，可靠的光学生物传感工具，用于检测重金属[Hg（II），As（V）或Cd（II）]和农药[2,4-二氯苯氧基乙酸（2,4- D）]在水系统中。用于检测Hg（II），As（V），Cd（II）和2,4-D的浓度范围分别为2-32 ppm，4-128 ppm，16-512 ppm和100-600 ppm。相应的灵敏度阈值为2.0 ppm，4.0 ppm，16.0 ppm和100 ppm。抑制常数（K_d）（或EC_（20））值的比较表明，敏感性阈值的等级为Hg（II）＞ As（V）＞ Cd（II）＞ 2,4-D。此外，检测重金属和农药所需的时间少于30分钟。使用生物发光抑制方法，可以预测重金属和农药的浓度。

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  • 来源
    《Journal of Hazardous Materials》 |2012年第30期|p.114-123|共10页
  • 作者

    Rajeev Ranjan; Navin K. Rastogi; M.S. Thakur;

  • 作者单位

    Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute (A Constituent Laboratory of Council of Scientific and Industrial Research, India), Mysore 570020, India;

    Food Engineering Department, Central Food Technological Research Institute (A Constituent Laboratory of Council of Scientific and Industrial Research, India), Mysore 570020, India;

    Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute (A Constituent Laboratory of Council of Scientific and Industrial Research, India), Mysore 570020, India;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    P. leiognathi; immobilization; biophotonic bead; environmental toxicants; bioluminescence inhibition;

    机译：P. leiognathi;固定生物光子珠环境毒物;生物发光抑制;

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