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首页> 外文期刊>Journal of Huazhong University of Science and Technology >Changes of Survivin mRNA and Protein Expression during Paclitaxel Treatment in Breast Cancer Cells
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Changes of Survivin mRNA and Protein Expression during Paclitaxel Treatment in Breast Cancer Cells

机译：紫杉醇治疗乳腺癌细胞中Survivin mRNA和蛋白表达的变化

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摘要

In order to investigate the role of antiapoptosis gene, survivin in the resistance to palci-taxel, the expression of survivin mRNA and protein in the process of paclitaxel treatment in breast cancer cell line MCF-7 was detected. MCF-7 cells were incubated with paclitaxel at different concentrations. The growth inhibition rate of MCF-7 was investigated by tetrazolium bromide (MTT) colorimetry. The change of apoptosis was detected by Annexin-V/PI methods. The changes in the expression of survivin mRNA and protein were studied by reverse transcription polymerase chain reaction (RT-PCR) and Western-blot assay respectively. The growth inhibition rate of MCF-7 was increased in a concentration- and time-dependent manner. Paclitaxel of higher concentration could effectively induce apoptosis in MCF-7 cells after 48 h, while the expression of survivin was increased at early time (within 6 h) and decreased after 24 h regardless of treatment concentrations of paclitaxel. It suggested that tumor cells might evade the paclitaxel-induced cell cycle arrest and apoptosis by increasing the level of survivin at early treatment time.
机译：为了研究抗凋亡基因survivin在对紫杉醇的抗性中的作用，在乳腺癌细胞株MCF-7中检测紫杉醇治疗过程中survivin mRNA和蛋白的表达。 MCF-7细胞与紫杉醇以不同浓度孵育。 MCF-7的生长抑制率通过溴化四唑（MTT）比色法进行研究。用膜联蛋白-V / PI法检测细胞凋亡的变化。分别通过逆转录聚合酶链反应（RT-PCR）和Western-blot法研究了survivin mRNA和蛋白表达的变化。 MCF-7的生长抑制率以浓度和时间依赖性方式增加。高浓度紫杉醇可在48 h后有效诱导MCF-7细胞凋亡，而不论紫杉醇的治疗浓度如何，survivin的表达在早期（6 h内）增加，而在24 h后降低。这表明肿瘤细胞可能通过在早期治疗时增加survivin的水平来逃避紫杉醇诱导的细胞周期停滞和凋亡。

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