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首页> 外文期刊>Journal of Huazhong University of Science and Technology >Expression of SODD and P65 in ALL of Children and Its Relationship with Chemotherapeutic Drugs
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Expression of SODD and P65 in ALL of Children and Its Relationship with Chemotherapeutic Drugs

机译：儿童ALL中SODD和P65的表达及其与化学治疗药物的关系

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摘要

The expression of silience of death domains (SODD) and its clinical significance and relationship with phospho-NF-κB-p65 proteins in bone marrow cells of childhood acute lymphoblastic leukaemia (ALL) were explored, and the expression of SODD and phospho-NF-κB-p65 in Jurkat cells treated with chemotherapeutic drugs was detected in order to find a new chemotherapeutic target. The expression of SODD and phospho-NF-κB-p65 proteins in bone marrow cells was detected by immunohistochemistry in 25 children with ALL. The apoptosis rate was measured by An-nexin-V-Fluorescence/PI double-labeling flow cytometry and the expression of SODD and phos-pho-NF-κB-p65 proteins determined by Western blotting in the Jurkat cells. It was found that the expression of SODD and active P65 in ALL was significantly higher than that in normal control group (P< 0.05). The expression of the SODD and phospho-NF-κB-p65 proteins in the high-risk (HR) group was significantly higher than that in the standard-risk (SR) group (P< 0.05). The Pearson rank correlation analysis revealed that there was a positive correlation between SODD and phospho-NF-κB-p65 expression (P< 0.01, r=0.69). VCR could effectively induce the apoptosis of Jurkat cells, and down-regulate the expression of SODD and phospho-NF-κB-p65 proteins in a time-dependent manner, but DNR could not down-regulate the expression of SODD effectively. It was concluded that SODD may be closely related to the clinical classification and prognosis of ALL in children. The expression of SODD and phospho-NF-κB-p65 had a definite synergistic relationship with the onset and development of ALL. VCR could down-regulate the expression of SODD and inhibit the NF-κB activation, which could recover the sensibility of apoptosis in leukemic cells.
机译：研究了儿童急性淋巴细胞白血病（ALL）骨髓细胞中死亡域复原力（SODD）的表达及其临床意义及其与磷酸化NF-κB-p65蛋白的关系，并探讨了其的表达检测化疗药物处理后的Jurkat细胞中的κB-p65，以寻找新的化疗靶点。用免疫组织化学方法检测25例ALL患儿骨髓中SODD和磷酸化NF-κB-p65蛋白的表达。通过An-nexin-V-荧光/ PI双标记流式细胞术测量细胞凋亡率，并通过Western印迹法测定Jurkat细胞中SODD和phos-pho-NF-κB-p65蛋白的表达。发现ALL中SODD和活性P65的表达明显高于正常对照组（P <0.05）。高危（HR）组SODD和磷酸化NF-κB-p65蛋白的表达明显高于标准高危（SR）组（P <0.05）。皮尔逊秩相关分析表明，SODD与磷酸化NF-κB-p65表达呈正相关（P <0.01，r = 0.69）。 VCR可以有效地诱导Jurkat细胞凋亡，并以时间依赖性方式下调SODD和磷酸化NF-κB-p65蛋白的表达，而DNR则不能有效地下调SODD的表达。结论是SODD可能与儿童ALL的临床分类和预后密切相关。 SODD和磷酸化NF-κB-p65的表达与ALL的发生和发展有着明确的协同关系。 VCR可以下调SODD的表达，抑制NF-κB的活化，从而恢复白血病细胞凋亡的敏感性。

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