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首页> 外文期刊>Journal of Huazhong University of Science and Technology >Construction of HA-l-DC Nucleic-acid Vaccine and Induction of Specific Cytotoxic T Lymphocytes
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Construction of HA-l-DC Nucleic-acid Vaccine and Induction of Specific Cytotoxic T Lymphocytes

机译：HA-1-DC核酸疫苗的构建及特异性细胞毒性T淋巴细胞的诱导

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An HA-1-DC nucleic-acid vaccine was constructed to induce anti-leukemia effect after hematopoietic stem cell transplantation (HSCT). DCs were generated from HSCT donors in vitro, and its immunologic activity was assayed by using flow cytometry and mixed lymphocytes reaction. HA-1 gene was electroporated into the cultured DCs to construct a DC nucleic-acid vaccine. After transfection for 48 h, the expression of HA-1 protein could be detected by using Western blot. The DCs were cultured with syngenic lymphocytes to induce specific cytotoxic T lymphocytes (CTLs). The cytoxicity of the CTLs was detected by LDH assay. The results showed that The DCs derived from peripheral blood monocytes (PBMCs) expressed the phenotype of DCs, and were effective in stimulating proliferation of the allogenic lymphocytes. After electroporating for 48—h, HA-1 protein was detected by using Western blot. The cytotoxity of inducing CTLs was higher than the control group. It was suggested that minor histocompatibility antigen HA-1 could be considered as a target of immunotherapy against leukemia after HSCT.
机译：构建了HA-1-DC核酸疫苗，以在造血干细胞移植（HSCT）后诱导抗白血病作用。从HSCT供体体外产生DC，并通过流式细胞术和混合淋巴细胞反应测定其免疫活性。将HA-1基因电穿孔到培养的DC中，以构建DC核酸疫苗。转染48 h后，采用Western blot检测HA-1蛋白的表达。用同基因淋巴细胞培养DC以诱导特异性的细胞毒性T淋巴细胞（CTL）。通过LDH测定法检测CTL的细胞毒性。结果表明，来自外周血单核细胞（PBMC）的DC表达DC的表型，并有效刺激同种异体淋巴细胞的增殖。电穿孔48小时后，使用Western blot检测HA-1蛋白。诱导CTLs的细胞毒性高于对照组。有人提出，小的组织相容性抗原HA-1可被认为是HSCT后针对白血病的免疫疗法的靶标。

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