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首页> 外文期刊>Journal of Huazhong University of Science and Technology >Knockdown of Survivin Expression by siRNA Induces Apoptosis of Hepatocellular Carcinoma Cells
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Knockdown of Survivin Expression by siRNA Induces Apoptosis of Hepatocellular Carcinoma Cells

机译：siRNA抑制Survivin表达诱导肝癌细胞的凋亡

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摘要

Survivin a newly identified member of IAP family, is a powerful apoptosis-inhibiting factor. It is expressed in embryonic tissues as well as in the majority of human cancers, but not in most normal adult tissues. The cancer-specific expression of survivin makes it a potential target for cancer treatment. A survivin-specific small inhibitory RNA (siRNA) was introduced into hepatocellular carcinoma cells to investigate its effect on cancer cell apoptosis, growth and sensitivity to che-motherapeutic drugs. It was found that expressions of survivin protein and proliferation index (PI) in siRNA groups were significantly decreased, the apoptosis index (AI) of siRNA groups was significantly higher than those of others groups, and the growth inhibition rate (GIR) of chemotherapeutic drugs in siRNA groups were significantly higher than those of other groups. Our study suggests that the expression of survivin may be significantly decreased in hepG2 cell after siRNA transfection. siRNA targeting survivin could induce cell apoptosis, inhibit cell proliferation and sensitize hepatocarcinoma cells to chemotherapy. Our findings provide preliminary evidence for the therapeutic use of survivin-targeted RNA interference for human tumors that express high levels of this molecule.
机译：Survivin是IAP家族的新成员，是一种强大的凋亡抑制因子。它在胚胎组织以及大多数人类癌症中表达，但在大多数正常的成人组织中不表达。 Survivin在癌症中的特异性表达使其成为癌症治疗的潜在靶标。将survivin特异性小抑制RNA（siRNA）引入肝细胞癌细胞中，以研究其对癌细胞凋亡，生长和对化疗药物敏感性的影响。发现siRNA组survivin蛋白的表达和增殖指数（PI）明显降低，siRNA组的凋亡指数（AI）明显高于其他组，化疗药物的生长抑制率（GIR） siRNA组中的siRNA显着高于其他组。我们的研究表明，siRNA转染后hepG2细胞中survivin的表达可能显着降低。靶向survivin的siRNA可以诱导细胞凋亡，抑制细胞增殖并使肝癌细胞对化疗敏感。我们的发现为survivin靶向RNA干扰物在表达高水平该分子的人类肿瘤中的治疗用途提供了初步证据。

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