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首页> 外文期刊>Journal of Imaging Science and Technology >Ink Jet Three-dimensional Digital Fabricationfor Biological Tissue Manufacturing: Analysis Of Alginate microgel Beads Produced By Ink Jet Droplets For Three dimensional Tissue Fabrication
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Ink Jet Three-dimensional Digital Fabricationfor Biological Tissue Manufacturing: Analysis Of Alginate microgel Beads Produced By Ink Jet Droplets For Three dimensional Tissue Fabrication

机译：用于生物组织制造的喷墨三维数字制造：用于三维组织制造的喷墨液滴产生的藻酸盐微凝胶珠的分析

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摘要

Ink jet technology has advantages such as high-resolution and a multicolor printing capability and has a good potential for computer-based three-dimensional (3D) fabrication. The authors have developed an ink jet 3D bioprinter to manufacture biologically viable 3D structures using living cells. They have developed an effective method for the fabrication of 3D hydrogel structures by using ink jet technology with a liquid aqueous gelating medium, which is essential in fabricating 3D structures with living cells. In the present study, they evaluated the feasibility of the ink jet approach for digital 3D biofabrication, analyzing the microgel beads produced by the ink jet droplets. The ink jet droplets of sodium alginate solution, which were ejected into CaCl_2 solution, gelled to form microgel beads. The resulting beads were analyzed by means of image-based particle analysis to show that homogeneously sized microgel beads were effectively produced. Ink jet 3D biofabrication has a high potential for effective digital fabrication with such homogeneous microgel beads and will provide promising approaches for sophisticated computer assisted tissue engineering.
机译：喷墨技术具有诸如高分辨率和多色打印能力的优点，并且对于基于计算机的三维（3D）制造具有良好的潜力。作者开发了一种喷墨3D生物打印机，以利用活细胞制造具有生物可行性的3D结构。他们已经开发出一种通过使用带有液态水性胶凝介质的喷墨技术来制造3D水凝胶结构的有效方法，这对于制造带有活细胞的3D结构至关重要。在本研究中，他们评估了喷墨方法用于数字3D生物制造的可行性，分析了由喷墨液滴产生的微凝胶珠。将藻酸钠溶液的喷墨液滴喷射到CaCl_2溶液中，使其胶凝形成微凝胶珠。通过基于图像的颗粒分析对所得的珠进行分析，以显示有效制备了均一尺寸的微凝胶珠。喷墨3D生物制造具有使用此类均质微凝胶珠进行有效数字制造的巨大潜力，并将为复杂的计算机辅助组织工程提供有希望的方法。

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