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Identification and differentiation of haze substances using Raman microspectroscopy

机译：使用拉曼微型光谱检查雾度物质的鉴定与分化

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摘要

The identification of turbidity or haze is an important part of brewery analytics. Haze can be caused by various issues throughout the brewing process and identifying the composition can pinpoint the origin. Haze analytics are commonly based on enzymatic or microscopic methods, which can be inaccurate or laborious. Raman microspectroscopy (RMS) presents a promising alternative for detecting haze particles. It is fast, easy to use and requires little sample preparation. Here, the applicability of RMS at 532 nm and 785 nm to identify potentially haze-forming particles has been evaluated. At 532 nm, measurements were taken with standard microscope slides. Due to the high fluorescent background of normal glass at an excitation wavelength of 785 nm, fused quartz microscope slides were used at this wavelength. Starch, arabinoxylan, cellulose, yeast beta-glucan, barley beta-glucan, gliadin, ferulic acid, proline, glutamine, calcium oxalate and PVPP were identified at 532 nm. The same substances when analysed at 785 nm resulted in problems with weak carbohydrate spectra of the beta-glucans and arabinoxylan. All the other substances could be analysed at 785 nm. Catechin, which produced fluorescence noise at 532 nm could be identified at 785 nm. Although there is an issue with the intense fluorescence noise of some beer components, Raman microspectroscopy has great potential in haze analysis and potentially in wider brewery analyses. (c) 2020 The Authors. Journal of the Institute of Brewing published by John Wiley & Sons Ltd on behalf of The Institute of Brewing & Distilling
机译：浊度或阴霾的识别是啤酒厂分析的重要组成部分。雾度可能是由整个酿造过程的各种问题引起的，并且识别组合物可以定位起源。雾度分析通常基于酶或微观方法，这可能是不准确或艰苦的。拉曼微型光谱学（RMS）呈现了检测雾度颗粒的有希望的替代方案。它快速，易于使用，需要少量样品制备。这里，已经评估了532nm和785nm处的rms的适用性，以鉴定形成潜在的雾度形成颗粒。在532nm处，用标准显微镜载玻片进行测量。由于常规玻璃的高荧光背景在激发波长为785nm，在该波长下使用熔融石英显微镜载玻片。淀粉，阿拉伯氧基，纤维素，酵母β-葡聚糖，大麦β-葡聚糖，胶质苷，阿魏酸，脯氨酸，谷氨酰胺，草酸钙和PVPP在532nm中鉴定。在785nm处分析时的相同物质导致β-葡聚糖和阿拉伯氧基的弱碳水化合物光谱有问题。所有其他物质都可以在785 nm处分析。在532nm处产生荧光噪声的儿茶素可以在785nm处鉴定。虽然存在一些啤酒组件的强烈荧光噪声存在问题，但拉曼微穴位术具有很大的雾度分析潜力，并且可能是更广泛的啤酒厂分析。 （c）2020作者。 John Wiley＆Sons Ltd在酿造和蒸馏研究所发表的酿造研究所

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  • 来源
    《Journal of the Institute of Brewing》 |2020年第4期|362-370|共9页
  • 作者

    Kahle Eva-Maria; Zarnkow Martin; Jacob Fritz;

  • 作者单位

    Tech Univ Munich Forschungszentrum Weihenstephan Brau & Lebensmitt Alte Akad 3 D-85354 Freising Weihenstephan Germany;

    Tech Univ Munich Forschungszentrum Weihenstephan Brau & Lebensmitt Alte Akad 3 D-85354 Freising Weihenstephan Germany;

    Tech Univ Munich Forschungszentrum Weihenstephan Brau & Lebensmitt Alte Akad 3 D-85354 Freising Weihenstephan Germany;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    beer haze; identification; Raman microspectroscopy;

    机译：啤酒阴霾;识别;拉曼微穴位检查;

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