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首页> 外文期刊>Journal of Biophotonics >Non-invasive identification of proteoglycans and chondrocyte differentiation state by Raman microspectroscopy
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Non-invasive identification of proteoglycans and chondrocyte differentiation state by Raman microspectroscopy

机译：拉曼光谱法无创地鉴定蛋白聚糖和软骨细胞分化状态

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Proteoglycans (PGs) are crucial extracellular matrix (ECM) components that are present in all tissues and organs. Pathological remodeling of these macromolecules can lead to severe diseases such as osteoarthritis or rheumatoid arthritis. To date, PG-associated ECM alterations are routinely diagnosed by invasive analytical methods. Here, we employed Raman microspectroscopy, a laser-based, marker-free and non-destructive technique that allows the generation of spectra with peaks originating from molecular vibrations within a sample, to identify specific Raman bands that can be assigned to PGs within human and porcine cartilage samples and chondrocytes. Based on the non-invasively acquired Raman spectra, we further revealed that a prolonged in vitro culture leads to phenotypic alterations of chondrocytes, resulting in a decreased PG synthesis rate and loss of lipid contents. Our results are the first to demonstrate the applicability of Raman microspectroscopy as an analytical and potential diagnostic tool for non-invasive cell and tissue state monitoring of cartilage in biomedical research. ((c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
机译：蛋白聚糖（PG）是存在于所有组织和器官中的重要细胞外基质（ECM）组件。这些大分子的病理重塑可能导致严重的疾病，例如骨关节炎或类风湿关节炎。迄今为止，与PG相关的ECM改变通常通过侵入性分析方法进行诊断。在这里，我们采用了拉曼显微光谱技术（一种基于激光的，无标记的无损技术），该技术可生成具有源自样品内分子振动的峰的光谱，以识别可分配给人和人体内PG的特定拉曼谱带。猪软骨样品和软骨细胞。基于无创获得的拉曼光谱，我们进一步揭示，长时间的体外培养会导致软骨细胞的表型改变，从而导致PG合成速率降低和脂质含量降低。我们的结果是第一个证明拉曼显微技术作为生物医学研究中非侵入性细胞和组织软骨状态监测的分析和潜在诊断工具的适用性。 （（c）2013 WILEY-VCH Verlag GmbH＆Co.KGaA，Weinheim）

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