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首页> 外文期刊>Journal of the Institute of Brewing >Enhancement of urea uptake in Chinese rice wine yeast strain N85 by the constitutive expression of DUR3 for ethyl carbamate elimination
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Enhancement of urea uptake in Chinese rice wine yeast strain N85 by the constitutive expression of DUR3 for ethyl carbamate elimination

机译：通过DUR3的本构表达消除氨基甲酸乙酯增强中国黄酒酵母N85菌株的尿素吸收。

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摘要

Most of the fermented alcoholic beverages, particularly Chinese rice wine, contain the potentially human carcinogenic compound ethyl carbamate (EC). As a major EC precursor in Chinese rice wine, urea in fermentations can be transported into the yeast cell by urea permease and finally metabolized by urea carboxylase and allophanate hydrolase in vivo. To eliminate EC in Chinese rice wines, the present study constructed high urea uptake yeast strains N1-D, N2-D and N-D, by introducing a strong promoter (PGK1p) into the urea permease gene (DUR3) of the industrial Chinese rice wine yeast N85, and by the restoration of the URA3 gene at the same time. With these self-cloned, high urea uptake strains, the urea and EC in the terminal Chinese rice wine samples were reduced to different extents. With two copies of overexpressed DUR3, the N-D strain could reduce the urea and the EC by 53.4 and 26.1%, respectively. No difference in fermentation characteristics was found between the engineered strains and the parental industrial yeast strain N85. These results could help to optimize the genetic manipulation strategy for EC elimination in Chinese rice wine production. Copyright (c) 2015 The Institute of Brewing & Distilling
机译：大多数发酵酒精饮料，特别是中国黄酒，都含有潜在的人类致癌化合物氨基甲酸乙酯（EC）。作为中国黄酒中主要的EC前体，发酵过程中的尿素可以通过尿素渗透酶转运到酵母细胞中，最后在体内被尿素羧化酶和脲基甲酸酯水解酶代谢。为了消除中国黄酒中的EC，本研究通过将强启动子（PGK1p）引入中国工业黄酒酵母的尿素透过酶基因（DUR3）中，构建了高尿素摄取酵母菌株N1-D，N2-D和ND。 N85，并同时还原URA3基因。通过这些自克隆的高尿素吸收菌株，中国黄酒末样品中的尿素和EC降低了不同程度。使用两份过表达的DUR3，N-D菌株可以分别降低尿素和EC的53.4和26.1％。在工程菌株和亲代工业酵母菌株N85之间没有发现发酵特性的差异。这些结果可能有助于优化中国黄酒生产中消除EC的基因操作策略。版权所有（c）2015酿酒与蒸馏研究所

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  • 来源
    《Journal of the Institute of Brewing》 |2015年第2期|257-261|共5页
  • 作者

    Li Xiaomin; Shen Chao; Wu Dianhui; Lu Jian; Chen Jian; Xie Guangfa;

  • 作者单位

    Jiangnan Univ, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China|Jiangnan Univ, Natl Engn Lab Cereal Fermentat Technol, Wuxi 214122, Peoples R China|Jiangnan Univ, Sch Biotechnol, Wuxi 214122, Peoples R China;

    Jiangnan Univ, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China|Jiangnan Univ, Natl Engn Lab Cereal Fermentat Technol, Wuxi 214122, Peoples R China|Jiangnan Univ, Sch Biotechnol, Wuxi 214122, Peoples R China;

    Jiangnan Univ, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China|Jiangnan Univ, Natl Engn Lab Cereal Fermentat Technol, Wuxi 214122, Peoples R China|Jiangnan Univ, Sch Biotechnol, Wuxi 214122, Peoples R China;

    Jiangnan Univ, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China|Jiangnan Univ, Natl Engn Lab Cereal Fermentat Technol, Wuxi 214122, Peoples R China|Jiangnan Univ, Sch Biotechnol, Wuxi 214122, Peoples R China|Jiangnan Univ Suqian, Ind Technol Res Inst, Suqian 223800, Peoples R China;

    Jiangnan Univ, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China|Jiangnan Univ, Natl Engn Lab Cereal Fermentat Technol, Wuxi 214122, Peoples R China|Jiangnan Univ, Sch Biotechnol, Wuxi 214122, Peoples R China;

    Jiangnan Univ, Sch Biotechnol, Wuxi 214122, Peoples R China|China Shaoxing Rice Wine Grp Co Ltd, Natl Engn Res Ctr Chinese Rice Wine, Shaoxing 312000, Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Chinese rice wine; yeast; urea uptake; ethyl carbamate; DUR3;

    机译：中国黄酒;酵母;尿素摄取;氨基甲酸乙酯;DUR3;

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