首页> 外文期刊>Journal of the Institute of Brewing >The Variation of β-amylase Activity and Protein Fractions in Barley Grains as Affected by Genotypes and Post-anthesis Temperatures
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The Variation of β-amylase Activity and Protein Fractions in Barley Grains as Affected by Genotypes and Post-anthesis Temperatures

机译:基因型和花后温度对大麦籽粒β-淀粉酶活性和蛋白质组分的影响

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摘要

The variation of β-amylase activity and protein fractions in barley grains was evaluated using 148 barley genotypes grown in the field and two cultivars under in vitro culture with two temperature treatments during grain development. The results showed that there was significant genotypic variation in β-amylase activity and protein fraction content. Regression analysis indicated that β-amylase activity was positively correlated with total protein and the level of each of the protein fractions, with the correlation coefficient between β-amylase activity and hordein content being the highest. Furthermore, higher post-anthesis temperatures (32/26℃, dayight) significantly enhanced β-amylase activity and protein fraction content, presumably as a result of reduced starch content. Albumin and glutelin were the least and most affected, respectively, in comparison with the plants under lower temperature (22/16℃). Temperature post-anthesis also influenced the morphology of the starch A granule and the number of B granules, suggesting the altered starch structure may also be a reason for deteriorated malting quality under high temperatures.
机译:使用在田间生长的148个大麦基因型和两个在培养过程中经过两种温度处理的体外培养的大麦品种,评估了大麦谷粒中β-淀粉酶活性和蛋白质组分的变化。结果表明,β-淀粉酶活性和蛋白质组分含量存在明显的基因型差异。回归分析表明,β-淀粉酶活性与总蛋白和各个蛋白组分的水平呈正相关,β-淀粉酶活性与大麦醇溶蛋白含量之间的相关系数最高。此外,较高的花后温度(32/26℃,昼/夜)显着提高了β-淀粉酶活性和蛋白质组分含量,这可能是淀粉含量降低的结果。与较低温度(22/16℃)下的植物相比,白蛋白和谷蛋白的影响分别最小和最大。花后温度也影响淀粉A颗粒的形态和B颗粒的数量,这表明淀粉结构的改变也可能是高温下麦芽品质下降的原因。

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