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首页> 外文期刊>Journal of the Institute of Brewing >Development of a 'Stress Model' Fermentation System for Fuel Ethanol Yeast Strains
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Development of a 'Stress Model' Fermentation System for Fuel Ethanol Yeast Strains

机译：燃料乙醇酵母菌“压力模型”发酵系统的开发

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摘要

During industrial scale fuel ethanol fermentations, yeast encounters a multitude of stress factors that impose constraints on growth and fermentative metabolism. These stresses include high sugar concentration, elevated temperature, high ethanol concentrations, low external pH and the weak organic acids lactic and acetic. Yeast strains which are tolerant to these stresses and able to synthesize high ethanol concentrations in their presence would be most desirable for use in industrial scale fuel ethanol production. In this study, a "stress model" fermentation system was developed as a tool to screen candidate yeast strains for relative stress resistance. The stress model was designed on the basis that the degree of ethanol produced by a particular strain would be indicative of the stress resistance of that particular strain. Eight strains of Saccharomyces cerevisiae, each with different backgrounds and fermentative capabilities, were screened for relative stress resistance using the stress model. The results obtained indicate that the sum of the stress factors in the stress model exceeded the tolerance level of most of the strains screened (approximately 40%). Two strains in particular, J006 and A007, displayed superior fermentative performance and produced significantly (P < 0.01) higher final ethanol concentrations when compared to the other strains.
机译：在工业规模的燃料乙醇发酵过程中，酵母会遇到多种压力因素，这些因素会限制生长和发酵代谢。这些压力包括高糖浓度，高温，高乙醇浓度，低外部pH值以及弱有机酸乳酸和醋酸。在工业规模的燃料乙醇生产中，最需要能够耐受这些压力并能够在其存在下合成高乙醇浓度的酵母菌株。在这项研究中，开发了“压力模型”发酵系统，作为筛选候选酵母菌株相对抗性的工具。基于特定菌株产生的乙醇的程度将指示该特定菌株的抗应力性，来设计应力模型。使用应激模型筛选了八种酿酒酵母菌株，每个菌株具有不同的背景和发酵能力，以进行相对应激抗性的筛选。所得结果表明，应力模型中应力因子的总和超过了大多数筛选菌株的耐受水平（约40％）。与其他菌株相比，两种菌株，特别是J006和A007，表现出卓越的发酵性能，并产生较高的最终乙醇浓度（P <0.01）。

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