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首页> 外文期刊>Journal of the Institute of Brewing >Modification of the Methods for the Extraction of Carboxypeptidase and Proteinase Activities from Sorghum Malts
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Modification of the Methods for the Extraction of Carboxypeptidase and Proteinase Activities from Sorghum Malts

机译：高粱麦芽中羧肽酶和蛋白酶活性提取方法的改进

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摘要

The effects of two extractants on the activities of carboxypeptid-ase and proteinase enzymes from three sorghum varieties germinated for 3 and 5 days were studied. In all three varieties, cys-teine hydrochloride (Cyst. HC1) proved a better extractant than 2-mercaptoethanol (2-ME) as shown by the increase in carboxy-peptidase and proteinase activities with 5-day malts. A three-way analysis of variance (ANOVA) on the effect of germination time, extractant and variety on the Carboxypeptidase activities of the three malts showed that all the factors plus their possible four-way interactions highly significantly (P < 0.001) affected the ex-tractability/activity of the enzyme. However, while the three factors had a highly significant (P < 0.001) effect on the proteinase activity, one of their four-way interactions, specifically, extract-ant/variety, had no significant effect. This suggests that, unlike the Carboxypeptidase enzyme, the effectiveness of the extractants in enhancing the extractability/activity of the proteinase enzyme in all the sorghum malt samples was amongst other factors, not variety-dependent.
机译：研究了两种萃取剂对发芽3天和5天的3个高粱品种羧肽酶和蛋白酶活性的影响。在所有三个品种中，半胱氨酸盐酸盐（Cyst。HCl）证明比2-巯基乙醇（2-ME）更好的萃取剂，这是随着5天麦芽中羧肽酶和蛋白酶活性的增加所证明的。对发芽时间，提取剂和品种对三种麦芽羧肽酶活性的影响进行方差分析（ANOVA）的三方分析表明，所有因素及其可能的四向相互作用均极显着（P <0.001） -酶的可延展性/活性。但是，尽管这三个因素对蛋白酶活性具有极显着的影响（P <0.001），但它们的四向相互作用之一，特别是提取剂/品种，却没有显着影响。这表明，与羧肽酶不同，萃取剂在提高所有高粱麦芽样品中蛋白酶的可萃取性/活性方面的有效性是其他因素之一，与品种无关。

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