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首页> 外文期刊>Journal oflaser Applications >Use of dispersive Raman spectroscopy to detect the cytotoxic action of viscum album in adenocarcinoma of colon
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Use of dispersive Raman spectroscopy to detect the cytotoxic action of viscum album in adenocarcinoma of colon

机译：色散拉曼光谱法检测粘液相册在结肠腺癌中的细胞毒性作用

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摘要

In the past decades it was noticed that there was a gradual increase in neoplasia diagnostics in animals, which are attributed to the longevity achieved by efficient vaccines, antibiotics, diagnostics, and food quality. The aliphatic oncology uses chemotherapies that are citostatic association, citostatic antibiotics, antimetabolic citostatics, and antimetabolic hormones in tumor treatment. Later on, the oncology introduced the imunomodulated antineoplasics from vegetals which belongs the viscum album. This study verified the efficiency of the extract of the injectable viscum album with concentrations D3 (0.1 μg/mL), D6 (0.1 ng/mL), D9 (0.1 × 10~(-3) ng/mL), D12 (0.1 × 10~(-6) ng/mL), and D30 (0.1 × 10~(-24) ng/mL) in culture of Caco-2 cells in vitro evaluated by correlating the spectral information obtained by dispersive Raman spectroscopy at 830 nm excitation with the cell viability determined by the Mitochondrial Activity (MTT) assay test. Results of MTT indicated that viscum album acted in the mitochondrial activity by reducing cellular viability in the Caco-2 cells in the concentrations of D3, D6, and D9. It was found that there was a good correlation of the Raman spectrum analyzed by principal components analysis, with the values of the MTT, and such result indicated that Raman could constitute in a sensitive tool for the detection of changes in the mitochondrial activity of Caco-2 cells.
机译：在过去的几十年中，人们注意到动物的瘤形成诊断逐渐增多，这归因于有效疫苗，抗生素，诊断方法和食品质量的长效性。脂族肿瘤学在肿瘤治疗中使用化学疗法，即化学疗法联合，化学疗法抗生素，抗代谢的抗静电药和抗代谢激素。后来，肿瘤学从属于粘液相册的植物中引入了免疫调节的抗肿瘤药。这项研究验证了浓度D3（0.1μg/ mL），D6（0.1 ng / mL），D9（0.1×10〜（-3）ng / mL），D12（0.1×通过在830 nm激发下通过分散拉曼光谱获得的光谱信息进行相关联评估，体外评估了Caco-2细胞培养物中的10〜（-6）ng / mL）和D30（0.1×10〜（-24）ng / mL）。通过线粒体活性（MTT）分析测试确定的细胞活力。 MTT的结果表明，在D3，D6和D9浓度下，粘胶相册通过降低Caco-2细胞中的细胞活力来发挥线粒体活性。发现通过主成分分析分析的拉曼光谱与MTT值之间具有良好的相关性，并且该结果表明拉曼可以构成检测Caco-的线粒体活性变化的灵敏工具。 2个单元格。

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  • 来源
    《Journal oflaser Applications》 |2009年第4期|p.163-168|共6页
  • 作者

    Daniela Franco Lopes; Landulfo Silveira Jr.; Marcelo Nobuo Sibata; Aloisio Cunha de Carvalho; Cristina Pacheco-Soares; Jamil Saade; Marcos Tadeu T. Pacheco;

  • 作者单位

    Institute of Research and Development (IP&D), Universidade do Vale do Paraiba (UNIVAP), Av. Shishima Hifumi, 2911 Urbanova, 12244-000 Sao Jose dos Campos, SP, Brazil;

    Group of Biomedical Engineering, Universidade Camilo Castelo Branco (UNICASTELO), Rod. Pres. Dutra, Km 138, Eugenio de Melo, 12247-004 Sao Jose dos Campos, SP, Brazil;

    Institute of Research and Development (IP&D), Universidade do Vale do Paraiba (UNIVAP), Av. Shishima Hifumi, 2911 Urbanova, 12244-000 Sao Jose dos Campos, SP, Brazil;

    Institute of Research and Development (IP&D), Universidade do Vale do Paraiba (UNIVAP), Av. Shishima Hifumi, 2911 Urbanova, 12244-000 Sao Jose dos Campos, SP, Brazil;

    Institute of Research and Development (IP&D), Universidade do Vale do Paraiba (UNIVAP), Av. Shishima Hifumi, 2911 Urbanova, 12244-000 Sao Jose dos Campos, SP, Brazil;

    Institute of Research and Development (IP&D), Universidade do Vale do Paraiba (UNIVAP), Av. Shishima Hifumi, 2911 Urbanova, 12244-000 Sao Jose dos Campos, SP, Brazil;

    Group of Biomedical Engineering, Universidade Camilo Castelo Branco (UNICASTELO), Rod. Pres. Dutra, Km 138, Eugenio de Melo, 12247-004 Sao Jose dos Campos, SP, Brazil;

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  • 正文语种 eng
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  • 关键词

    injectable homeopatic medicine; neoplasm; colon; cell culture; MTTt assay; dispersive raman spectroscopy; principal components analysis (PCA);

    机译：注射顺势疗法药物;肿瘤;结肠;细胞培养;MTT分析;色散拉曼光谱主成分分析（PCA）;

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