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首页> 外文期刊>Journal of magnetism and magnetic materials >In situ preparation of high relaxivity iron oxide nanoparticles by coating with chitosan: A potential MRI contrast agent useful for cell tracking
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In situ preparation of high relaxivity iron oxide nanoparticles by coating with chitosan: A potential MRI contrast agent useful for cell tracking

机译：通过壳聚糖涂层原位制备高弛豫性氧化铁纳米粒子：一种潜在的MRI造影剂，可用于细胞跟踪

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摘要

Iron oxide nanocrystals are of considerable interest in nanoscience and nanotechnology because of their nanoscale dimensions, nontoxic nature, and superior magnetic properties. Colloidal solutions of magnetic nanoparticles (ferrofluids) with a high magnetite content are highly desirable for most molecular imaging applications. In this paper, we present a method for in situ coating of superparamagnetic iron oxide (SPIO) with chitosan in order to increase the content of magnetite. Iron chloride salts (Fe~(3+) and Fe~(2+)) were directly coprecipitated inside a porous matrix of chitosan by Co-60 γ-ray irradiation in an aqueous solution of acetic acid. Following sonication, iron oxide nanoparticles were formed inside the chitosan matrix at a pH value of 9.5 and a temperature of 50 ℃ The [Fe~(3+)]:[Fe~(2+)]:[NH_4OH] molar ratio was 1.6:1:15.8. The final ferrofluid was formed with a pH adjustment to approximately 2.0/3.0, alongside with the addition of mannitol and lactic acid. We subsequently characterized the particle size, the zeta potential, the iron concentration, the magnetic contrast, and the cellular uptake of our ferrofluid. Results showed a z-average diameter of 87.2 nm, a polydispersity index (PDI) of 0.251, a zeta potential of 47.9 mV, and an iron concentration of 10.4 mg Fe/mL. The MRI parameters included an R1 value of 22.0 mM~(-1) s~(-1), an R2 value of 202.6 mM~(-1) s~(-1), and a R2/R1 ratio of 9.2. An uptake of the ferrofluid by mouse macrophages was observed. Altogether, our data show that Co-60 y-ray radiation on solid chitosan may improve chitosan coating of iron oxide nanoparticles and tackle its aqueous solubility at pH 7. Additionally, our methodology allowed to obtain a ferrofluid with a higher content of magnetite and a fairly unimodal distribution of monodisperse clusters. Finally, MRI and cell experiments demonstrated the potential usefulness of this product as a potential MRI contrast agent that might be used for cell tracking.
机译：氧化铁纳米晶体因其纳米级尺寸，无毒性质和优越的磁性而在纳米科学和纳米技术中引起了极大的兴趣。具有高磁铁矿含量的磁性纳米颗粒（铁磁流体）的胶体溶液对于大多数分子成像应用来说是非常需要的。在本文中，我们提出了一种利用壳聚糖原位涂覆超顺磁性氧化铁（SPIO）的方法，以增加磁铁矿的含量。氯化铁盐（Fe〜（3+）和Fe〜（2+））在乙酸水溶液中通过Co-60γ射线辐照直接在壳聚糖的多孔基质中共沉淀。超声处理后，在pH值为9.5和温度为50℃的条件下，在壳聚糖基质中形成了氧化铁纳米颗粒。[Fe〜（3 +）]：[Fe〜（2 +）]：[NH_4OH]的摩尔比为1.6 ：1：15.8。形成最终的铁磁流体，将pH值调节至约2.0 / 3.0，同时添加甘露醇和乳酸。随后，我们表征了铁磁流体的粒径，ζ电位，铁浓度，磁对比和细胞吸收。结果显示，z平均直径为87.2 nm，多分散指数（PDI）为0.251，ζ电位为47.9 mV，铁浓度为10.4 mg Fe / mL。 MRI参数包括R1值为22.0 mM〜（-1）s〜（-1），R2值为202.6 mM〜（-1）s〜（-1），R2 / R1比为9.2。观察到小鼠巨噬细胞摄取铁磁流体。总而言之，我们的数据表明，固态壳聚糖上的Co-60 y射线辐射可能会改善氧化铁纳米颗粒的壳聚糖涂层并解决其在pH 7下的水溶性。此外，我们的方法允许获得具有更高磁铁矿含量和铁含量的铁磁流体。单分散簇的相当单峰分布。最后，MRI和细胞实验证明了该产品作为可能用于细胞追踪的MRI造影剂的潜在用途。

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  • 来源
    《Journal of magnetism and magnetic materials》 |2010年第2期|208-213|共6页
  • 作者

    Zei-Tsan Tsai; Jen-Fei Wang; Hsiao-Yun Kuo; Chia-Rui Shen; Jiun-Jie Wang; Tzu-Chen Yen;

  • 作者单位

    Molecular Imaging Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan;

    Molecular Imaging Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan;

    Molecular Imaging Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan Graduate Institute and Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan, Taiwan;

    Molecular Imaging Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan Graduate Institute and Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan, Taiwan;

    Molecular Imaging Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan Department of Medical Imaging and Radiological Sciences, Chang Gung University, Taoyuan, Taiwan;

    Molecular Imaging Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan Department of Nuclear Medicine, Chang Cung Memorial Hospital, Taoyuan, Taiwan;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    superparamagnetic iron oxide; chitosan; γ-Ray radiation; MRi contrast agent;

    机译：超顺磁性氧化铁壳聚糖γ射线辐射;MRi造影剂;

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