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首页> 外文期刊>Journal of materials science >Multiwall carbon nanotubes/polycaprolactone scaffolds seeded with human dental pulp stem cells for bone tissue regeneration
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Multiwall carbon nanotubes/polycaprolactone scaffolds seeded with human dental pulp stem cells for bone tissue regeneration

机译：植入人牙髓干细胞的多壁碳纳米管/聚己内酯支架用于骨组织再生

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摘要

Conventional approaches to bone regeneration rarely use multiwall carbon nanotubes (MWCNTs) but instead use polymeric matrices filled with hydroxyapatite, calcium phosphates and bioactive glasses. In this study, we prepared composites of MWCNTs/polycaprolactone (PCL) for bone regeneration as follows: (a) MWCNTs randomly dispersed on PCL, (b) MWCNTs aligned with an electrical field to determine if the orientation favors the growing of human dental pulp stem cells (HDPSCs), and (c) MWCNTs modified with beta-glycerol phosphate (BGP) to analyze its osteogenic potential. Raman spectroscopy confirmed the presence of MWCNTs and BGP on PCL, whereas the increase in crystallinity by the addition of MWCNTs to PCL was confirmed by X-ray diffraction and differential scanning calorimetry. A higher elastic modulus (608 +/- 4.3 MPa), maximum stress (42 +/- 6.1 MPa) and electrical conductivity (1.67 x 10(-7) S/m) were observed in non-aligned MWCNTs compared with the pristine PCL. Cell viability at 14 days was similar in all samples according to the live/dead assay, but the 21 day cell proliferation, measured by MTT was higher in MWCNTs aligned with BGP. Von Kossa and Alizarin red showed larger amounts of mineral deposits on MWCNTs aligned with BGP, indicating that at 21 days, this scaffold promotes osteogenic differentiation of HDPSCs.
机译：传统的骨骼再生方法很少使用多壁碳纳米管（MWCNT），而是使用填充有羟基磷灰石，磷酸钙和生物活性玻璃的聚合物基体。在这项研究中，我们制备了用于骨再生的MWCNTs /聚己内酯（PCL）复合材料，如下：（a）MWCNTs随机分散在PCL上，（b）MWCNTs与电场对准，以确定方向是否有利于人类牙髓的生长干细胞（HDPSC）和（c）用β-甘油磷酸酯（BGP）修饰的MWCNT，以分析其成骨潜力。拉曼光谱法证实了PCL上存在MWCNT和BGP，而X射线衍射和差示扫描量热法证实了通过向PCL中添加MWCNT来提高结晶度。与原始PCL相比，在未取向的MWCNT中观察到更高的弹性模量（608 +/- 4.3 MPa），最大应力（42 +/- 6.1 MPa）和电导率（1.67 x 10（-7）S / m）。 。根据活/死分析，所有样品在第14天的细胞生存力都相似，但是在与BGP对齐的MWCNT中，MTT测定的21天细胞增殖更高。冯·科萨（Von Kossa）和茜素红（Arizarin red）在与BGP对齐的MWCNT上显示出大量矿物质沉积，表明在21天时，该支架促进了HDPSC的成骨分化。

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  • 来源
    《Journal of materials science》 |2016年第2期|35.1-35.12|共12页
  • 作者

    Flores-Cedillo M. L.; Alvarado-Estrada K. N.; Pozos-Guillen A. J.; Murguia-Ibarra J. S.; Vidal M. A.; Cervantes-Uc J. M.; Rosales-Ibanez R.; Cauich-Rodriguez J. V.;

  • 作者单位

    Univ Autonoma San Luis Potosi, Fac Estomatol, Lab Ciencias Basicas, San Luis Potosi, Mexico;

    Univ Autonoma San Luis Potosi, Fac Estomatol, Lab Ciencias Basicas, San Luis Potosi, Mexico;

    Univ Autonoma San Luis Potosi, Fac Estomatol, Lab Ciencias Basicas, San Luis Potosi, Mexico;

    Univ Autonoma San Luis Potosi, Fac Ciencias, Dept Elect, San Luis Potosi, Mexico;

    CIACYT, Ctr Aplicac Radiac Infrarroja Energias Alternat &, San Luis Potosi, Mexico;

    Ctr Invest Cient Yucatan, Unidad Mat, Merida, Yucatan, Mexico;

    Univ Autonoma San Luis Potosi, Fac Estomatol, Lab Ciencias Basicas, San Luis Potosi, Mexico;

    Ctr Invest Cient Yucatan, Unidad Mat, Merida, Yucatan, Mexico;

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