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首页> 外文期刊>Journal of materials science >Effects of intermittent hydrostatic pressure magnitude on the chondrogenesis of MSCs without biochemical agents under 3D co-culture
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Effects of intermittent hydrostatic pressure magnitude on the chondrogenesis of MSCs without biochemical agents under 3D co-culture

机译：3D共培养下间歇性静水压力幅度对无生化剂的MSC软骨形成的影响

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摘要

Without using biochemical agents, in this study, we sought to investigate the potential of controlling the differentiation of mesenchymal stem cells (MSCs) into a specific cell type through the use of 3D co-culturing and mechanical stimuli. MSCs and primary cultured chondro-cytes were separately encapsulated into alginate beads, and the two types of beads were separated by a membrane. For the investigation a computer-controllable bioreactor was designed and used to engage intermittent hydrostatic pressure (IHP). Five different magnitudes (0.20, 0.10, 0.05, 0.02 MPa and no stimulation) of IHP were applied. The stimulation pattern was the same for all groups: 2 h/day for days starting at 24 h after seeding; 2 and 15 min cycles of stimulating and resting, respectively. Biochemical (DNA and GAG contents), histological (Alcian blue), and RT-PCR (Col II, SOX9, AGC) analyses were performed on days 1, 5, 10, and 20. The results from these analyses showed that stimulation with higher magnitudes of IHP (≥0.10 MPa) were more effective on the proliferation and differentiation of co-cultured MSCs. Together, these data demonstrate the potential of using mechanical stimulation and co-culturing for the proliferation and differentiation of MSCs, even without biochemical agents.
机译：在不使用生化试剂的情况下，本研究试图通过3D共培养和机械刺激来研究控制间充质干细胞（MSCs）分化为特定细胞类型的潜力。将MSC和原代培养的软骨细胞分别封装成藻酸盐珠，然后用膜将两种珠分离。为了进行研究，设计了一种计算机可控制的生物反应器，并将其用于间歇性静水压（IHP）。应用了五个不同大小的IHP（0.20、0.10、0.05、0.02 MPa，无刺激）。所有组的刺激方式均相同：播种后24小时开始的每天2小时/天；分别刺激和休息2分钟和15分钟。在第1、5、10和20天进行了生化（DNA和GAG含量），组织学（阿尔辛蓝）和RT-PCR（Col II，SOX9，AGC）分析。 IHP（≥0.10MPa）强度对共培养的MSC的增殖和分化更有效。总之，这些数据证明了即使没有生化试剂，也可以使用机械刺激和共培养来促进MSC的增殖和分化。

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  • 来源
    《Journal of materials science》 |2012年第11期|2773-2781|共9页
  • 作者

    Jae Young Jeong; So Hee Park; Ji Won Shin; Yun Gyeong Kang ? Ki-Ho Han; Jung-Woog Shin;

  • 作者单位

    Department of Biomedical Engineering, Inje University, 607 Obang-Dong, Gimhae, Gyeongnam, Republic of Korea;

    Department of Biomedical Engineering, Inje University, 607 Obang-Dong, Gimhae, Gyeongnam, Republic of Korea;

    Department of Biomedical Engineering, Inje University, 607 Obang-Dong, Gimhae, Gyeongnam, Republic of Korea,First Research Team/Institute of Aged Life Redesign/ Cardiovascular and Metabolic Disease Center/UHRC, Inje University, Gimhae, Gyongnam, Republic of Korea;

    School of Nano Engineering, Inje University, 607 Obang-Dong, Gimhae, Gyeongnam, Republic of Korea;

    Department of Biomedical Engineering, Inje University, 607 Obang-Dong, Gimhae, Gyeongnam, Republic of Korea;

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