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Enhanced cell affinity of the silk fibroin- modified PHBHHx material

机译：丝素蛋白修饰的PHBHHx材料的增强的细胞亲和力

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摘要

Cell affinity is one of the important issues required for developing tissue engineering materials. Although the poly(3-hydroxybutyrate-co-3-hydroxyhex-anoate) (PHBHHx) has been attractive for its controllable mechanical properties recent years, its cell affinity is still necessary to be improved for the requirements. For this purpose, the regenerated silk fibroin (SF) was coated on the PHBHHx films and its porous scaffolds. The mechanical test showed that SF-modified PHBHHx (SF/PHBHHx) film has a maximum tensile strength of 11.5 ± 0.5 MPa and elongation at break of 175 ± 5%. ATR-FTIR spectroscopy demonstrated that SF firmly attached on the scaffold by the hydrogen bonding interaction between SF and PHBHHx even flushed for 21 days in the phosphate-buffer saline (PBS) solution (pH = 7.4). In order to characterize the cell affinity of the SF-modified material, endothelial-like cell line ECV304 cells were seeded on the SF/PHBHHx films and its porous scaffolds. The histochemical analyses of cells stained by the hematoxylin and eosin (HE) as well asrncell nuclei stained by the 4',6-diamindine-2'-phenylindole (DAPI) demonstrated that cell attached and reached nearly 100% confluence on the SF/PHBHHx films when cultured for 4 days, which was much faster than that on the pure PHBHHx film. Moreover, the assay of cell activity by the 3-(4, 5-dimethyl thiazol -2-yl)-2, 5-diphenyl terazolium bromide (MTT) showed quantitatively that the number of cells on the SF/PHBHHx porous scaffolds was significant more than that on the unmodified ones after 4, 8, and 14 days culture, respectively. Scanning electron microscopy (SEM) revealed the similar results. Therefore, the SF-modified PHBHHx material is maybe a potential material applicable in the cardiovascular tissue engineering.
机译：细胞亲和力是开发组织工程材料所需的重要问题之一。尽管近年来，聚（3-羟基丁酸酯-co-3-羟基己酸酯）（PHBHHx）具有吸引人的机械性能，但仍需要改善其细胞亲和力。为此，将再生的丝素蛋白（SF）涂覆在PHBHHx膜及其多孔支架上。力学测试表明，SF改性的PHBHHx（SF / PHBHHx）膜的最大拉伸强度为11.5±0.5 MPa，断裂伸长率为175±5％。 ATR-FTIR光谱表明，通过SF和PHBHHx之间的氢键相互作用，SF牢固地附着在支架上，甚至在磷酸盐缓冲液（PBS）（pH = 7.4）溶液中冲洗了21天。为了表征SF修饰材料的细胞亲和力，将内皮样细胞系ECV304细胞接种在SF / PHBHHx膜及其多孔支架上。对苏木精和曙红（HE）染色的细胞以及4'，6-diamindine-2'-苯基吲哚（DAPI）染色的星形胶质细胞核的组织化学分析表明，细胞附着并在SF / PHBHHx上达到了近100％融合膜培养4天后，比纯PHBHHx膜快得多。此外，通过3-（4,5-二甲基噻唑-2-基）-2，5-二苯基溴化偶氮溴化铵（MTT）进行的细胞活性测定定量显示，SF / PHBHHx多孔支架上的细胞数量显着分别比培养4天，8天和14天后未修饰的多。扫描电子显微镜（SEM）显示了相似的结果。因此，SF修饰的PHBHHx材料可能是适用于心血管组织工程的潜在材料。

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  • 来源
    《Journal of materials science》 |2009年第8期|1743-1751|共9页
  • 作者

    Min Sun; Ping Zhou; Luan-Feng Pan; Shui Liu; Hua-Xiao Yang;

  • 作者单位

    The Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, People's Republic of China;

    The Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, People's Republic of China;

    Laboratory of Molecular Biology, Shanghai Medical College, Fudan University, Shanghai 200032, People's Republic of China;

    Laboratory of Molecular Biology, Shanghai Medical College, Fudan University, Shanghai 200032, People's Republic of China;

    The Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, People's Republic of China;

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