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首页> 外文期刊>Journal of materials science >Characterization and osteoblast-like cell compatibility of porous scaffolds: bovine hydroxyapatite and novel hydroxyapatite artificial bone
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Characterization and osteoblast-like cell compatibility of porous scaffolds: bovine hydroxyapatite and novel hydroxyapatite artificial bone

机译：多孔支架的表征和类成骨细胞相容性：牛羟基磷灰石和新型羟基磷灰石人造骨

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摘要

Three different porous scaffolds were tested. The first two were prepared by sintering bovine bone. The third scaffold was prepared using three-dimensional gel-lamination, a new rapid prototyping method, and was named as hydroxyapatite artificial bone.rnX-ray diffraction and Fourier transform infrared spec-troscopy analysis confirmed that the samples were mainly highly crystalline hydroxyapatite ceramics. Scanning electron microscopy and mercury intrusion porosimetry measurement showed that the pores were interconnected and pore sizes ranged from several microns to hundreds of microns.rnMouse osteoblast-like cells grown on the three scaffolds retained their characteristic morphology. Cell proliferation and differentiation, analyzed by methylthiazol tetrazolium (MTT) and alkaline phosphatase activity assays, were significantly higher on the hydroxyapatite artificial bone than on the other two scaffolds tested. All the scaffolds provided good attachment, proliferation and differentiation of bone cells.rnThese results indicate that the scaffolds have a favorable interaction with cells, they support cell growth and functions, and therefore these scaffolds may have great potential as bone substitutes. The three-dimensional gel-lamination method is proven to be an attractive process to design and fabricaternbone scaffolds with favorable properties, and therefore, has promising potential for bone repair applications.
机译：测试了三种不同的多孔支架。前两个是通过烧结牛骨制备的。第三种支架是使用新型快速成型方法三维凝胶层压法制备的，被称为羟基磷灰石人造骨.rnX射线衍射和傅里叶变换红外光谱分析证实样品主要是高结晶度的羟基磷灰石陶瓷。扫描电子显微镜和压汞法测量表明，孔是相互连通的，孔的大小从几微米到几百微米不等。在三个支架上生长的小鼠成骨细胞样细胞保持其特征形态。通过甲基噻唑四唑（MTT）和碱性磷酸酶活性分析进行分析，在羟基磷灰石人造骨上的细胞增殖和分化明显高于其他两个被测试的支架。所有这些支架都提供了良好的骨细胞附着，增殖和分化功能。这些结果表明，支架与细胞之间具有良好的相互作用，支持细胞的生长和功能，因此这些支架具有巨大的骨替代潜力。事实证明，三维凝胶层压法是设计和制造具有良好性能的后骨头支架的一种有吸引力的方法，因此，在骨修复应用中具有广阔的发展潜力。

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  • 来源
    《Journal of materials science》 |2006年第9期|815-823|共9页
  • 作者

    Yuan Gao; Wen-Ling Cao; Xiao-Yan Wang; Yan-Dao Gong; Jie-Mo Tian; Nan-Ming Zhao; Xiu-Fang Zhang;

  • 作者单位

    Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China;

    Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China;

    Institute of Nuclear Energy Technology, Tsinghua University, Beijing 100084, China;

    Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China;

    Institute of Nuclear Energy Technology, Tsinghua University, Beijing 100084, China;

    Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China;

    Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China;

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