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首页> 外文期刊>Journal of microbiology and biotechnology >Pigment Reduction to Improve Photosynthetic Productivity of Rhodobacter sphaeroides
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Pigment Reduction to Improve Photosynthetic Productivity of Rhodobacter sphaeroides

机译:减少色素以提高球形红细菌的光合生产力

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摘要

Improving the light utilization efficiency of photosynthetic cells in photobioreactors (PBRs) is a major topic in algal biotechnology. Accordingly, in the current study we investigated the effect and suitability of photosynthetic pigment reduction for improving light utilization efficiency. The light-harvesting complex II (LH-II) genes of Rhodobacter sphaeroides were removed to construct a mutant strain witii less pigment content. The mutant strain exhibited a slower growth rate than the wild-type under a low light intensity, while the mutant grew faster under a high light intensity. In addition, the specific absorption coefficient was lower in the mutant due to its reduced pigment content, thus it seemed that light penetrated deeper into its culture broth. However, the distance (light penetration depth) from the surface of the PBR to the compensation point did not increase, due to an increase in the compensation irradiance of the mutant strain. Experimental data showed that a reduced photosynthetic pigment content, which lessened the photoinhibition under high-intensity light, helped the volumetric productivity of photosynthetic microorganisms.
机译:在藻类生物技术中,提高光生物反应器(PBR)中光合细胞的光利用效率是一个主要课题。因此,在当前的研究中,我们研究了光合色素还原对提高光利用效率的影响和适用性。去除球形球形红细菌的光采复合物II(LH-II)基因,以构建具有较少色素含量的突变菌株。在低光照强度下,突变菌株的生长速率比野生型慢,而在高光照强度下,突变菌株的生长更快。另外,由于突变体的色素含量降低,其比吸收系数较低,因此似乎光更深地渗透到其培养液中。然而,由于突变菌株的补偿辐照度的增加,从PBR的表面到补偿点的距离(光穿透深度)没有增加。实验数据表明,减少的光合色素含量,减少了高强度光下的光抑制作用,有助于提高光合微生物的生产力。

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