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Oxygen-Dependent Production and Arrangements of the Photosynthetic Pigments in Intact Cells of Rhodobacter Sphaeroides

机译:球形红球细菌完整细胞中氧依赖的生产和光合色素的排列

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The development and re-arrangement of photosynthetic membrane of Rhodobacter sphaeroides were studied by absorption spectroscopy and fast induction of bacteriochlorophyll fluorescence when the cells grown under semiaerobic conditions were inoculated to aerobic medium and, after accommodation to aerobiosis, transferred to anaerobic conditions during constant illumination. After sudden transition from semiaerobic to aerobic cultures, the photochemical yield characterized by the relative variable fluorescence Fv/Fmax decreased from 0.70 gradually to 0.30, the photochemical rate constant dropped from 5·104 s-1 to 1·104 s-1 and the contribution of the peripheral antenna (LIE) to the RC-LH1 core complex became less significant. The aerobic growth is characterized by bleaching of the pigment system. The transfer to anaerobiosis resulted in photosynthetic growth with opposite tendency of the parameters and the initial values were restored after a couple of cell cycling periods of doubling time of ~4 h. The LH2 to LH1 ratio increased by a factor of 5. It was demonstrated that the fast fluorescence induction is a sensitive and non-invasing method to monitor the physiological state of the photosynthetic apparatus upon variation of the oxygen concentration of the culture.
机译:当将在半需氧条件下生长的细胞接种到需氧培养基中并适应氧合后,在恒定光照下转移到厌氧条件下,通过吸收光谱法和细菌叶绿素荧光的快速诱导研究了球形红球菌光合膜的发育和重排。从半有氧培养突然转变为有氧培养后,以相对可变荧光Fv / Fmax为特征的光化学产量从0.70逐渐下降至0.30,光化学速率常数从5·104 s-1降至1·104 s-1,并做出了贡献外围天线(LIE)与RC-LH1核心组件之间的距离变得不那么重要了。有氧生长的特征在于颜料体系的漂白。转移到厌氧菌导致光合作用的增长,具有相反的参数趋势,并且在约4小时的倍增时间的几个细胞周期后恢复了初始值。 LH2与LH1的比率增加了5倍。事实证明,快速荧光诱导是一种敏感且非侵入性的方法,可以根据培养物中氧气浓度的变化来监测光合作用装置的生理状态。

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