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首页> 外文期刊>Journal of nano research >Formation of Chitosan Nanoparticles Using Deacetylated Chitin Isolated from Freshwater Algae and Locally Synthesized Zeolite A and their Influence on Cancer Cell Growth
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Formation of Chitosan Nanoparticles Using Deacetylated Chitin Isolated from Freshwater Algae and Locally Synthesized Zeolite A and their Influence on Cancer Cell Growth

机译：从淡水藻类和局部合成的沸石A分离的脱乙酰壳多糖壳聚糖纳米粒子的形成及其对癌细胞生长的影响。

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This work reports the isolation and characterization of chitin from green algae using XRD, C-13 CP/MAS NMR, FTIR and Microscopy. The XRD diffraction pattern confirmed orthorhombic structure of the crystalline polysaccharide, whereas the FTIR spectra revealed strong absorption bands at 896.9 cm(-1) and 852 cm(-1) typical of C-H axial and C-H equatorial vibrations within the anomeric center of the glucopyranosicyclic moiety. Another strong absorption band was observed at 1039.9 cm-1 and was assigned to C-O-C, C-O stretching bands. The purity and structure of the deacetylated chitin was confirmed using C-13 NMR, showing overlapping peaks around 65 ppm assigned to both the sugar carbon at C2, as well as a methylene carbon at C6. An intense peak at 74 ppm is assigned to C3 and C5 with corresponding resonances at 81 and 104 ppm assigned to C4 and C1 respectively. Zeolite/Chitosan nanocomposites were synthesized by ionic cross-linking of chitosan with sodium tripolyphosphate. Chitosan nanoparticles and LTA of different concentrations were incubated with HeLa cancer cells to investigate their cytotoxicity effects. The exposure of the cells to chitosan nanoparticles resulted in a decreased in cell growth and this was concentration-dependent. Our results revealed the utility of locally available materials to produce new biodegradable nanoparticles to investigate their biological nanotoxicity.
机译：这项工作报告了使用XRD，C-13 CP / MAS NMR，FTIR和显微技术从绿藻中分离和鉴定几丁质。 XRD衍射图证实了结晶多糖的正交结构，而FTIR谱显示在吡喃吡喃并环部分异头中心内CH轴和CH赤道振动典型的896.9 cm（-1）和852 cm（-1）处有很强的吸收带。在1039.9 cm-1处观察到另一个强吸收带，并被指定为C-O-C，C-O拉伸带。使用C-13 NMR证实了脱乙酰基几丁质的纯度和结构，在C2处的糖碳和C6处的亚甲基碳均显示了约65 ppm的重叠峰。在74 ppm处有一个强烈的峰值分配给C3和C5，在81 ppm和104 ppm处有相应的谐振分别分配给C4和C1。通过壳聚糖与三聚磷酸钠的离子交联合成了沸石/壳聚糖纳米复合材料。将不同浓度的壳聚糖纳米颗粒和LTA与HeLa癌细胞孵育，以研究其细胞毒性作用。细胞暴露于壳聚糖纳米颗粒会导致细胞生长减少，这是浓度依赖性的。我们的结果表明，当地可得的材料可用于生产新的可生物降解的纳米颗粒，以研究其生物纳米毒性。

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