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首页> 外文期刊>Journal of Natural Medicines >DNA authentication of So-jutsu (Atractylodes lancea rhizome) and Byaku-jutsu (Atractylodes rhizome) obtained in the market based on the nucleotide sequence of the 18S–5.8S rDNA internal transcribed spacer region
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DNA authentication of So-jutsu (Atractylodes lancea rhizome) and Byaku-jutsu (Atractylodes rhizome) obtained in the market based on the nucleotide sequence of the 18S–5.8S rDNA internal transcribed spacer region

机译：基于18S–5.8S rDNA内部转录间隔区的核苷酸序列，在市场上获得的So-jutsu（Atractylodes lancea根状茎）和Byaku-jutsu（Atractylodes根状茎）的DNA鉴定

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摘要

The internal transcribed spacer 1 (ITS1) region between 18S and 5.8S rDNA was amplified from 25 crude drug specimens of So-jutsu (Atractylodes lancea rhizome) and 11 specimens of Byaku-jutsu (Atractylodes rhizome) obtained in the Chinese market. Based on the comparison of the nucleotide sequences of the PCR products with those determined in five Atractylodes species, we were able to identify the plant origins of these crude drugs. Of the 25 specimens of So-jutsu analyzed, 20 were identified to be derived from either A. chinensis, A. lancea or the interspecific hybrid between these two species, whereas five specimens were identified to be derived from A. japonica, i.e. Byaku-jutsu. In contrast, all of the specimens of Byaku-jutsu analyzed were derived from either A. ovata or A. japonica. A PCR-based method was established for the rapid identification and/or discrimination of So-jutsu and Byaku-jutsu without sequencing.
机译：从中国市场获得的25个So-jutsu（白术）生药标本和11个Byaku-jutsu（白术）标本中扩增了18S和5.8S rDNA之间的内部转录间隔区1（ITS1）区域。通过将PCR产物的核苷酸序列与五个白术物种中确定的核苷酸序列进行比较，我们能够确定这些粗制药物的植物来源。在经分析的25种柔术标本中，有20种被鉴定出源自中华曲霉，柳叶刀或这两个物种之间的种间杂种，而五种标本被鉴定出源自日本粳稻，即Byaku-柔术。相反，所分析的Byaku-jutsu的所有标本均来自卵形拟南芥或粳稻。建立了一种基于PCR的方法，无需进行测序即可快速鉴定和/或辨别So-jutsu和Byaku-jutsu。

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  • 来源
    《Journal of Natural Medicines》 |2006年第2期|149-156|共8页
  • 作者

    Yahong Guo; Kenji Kondo; Susumu Terabayashi; Yutaka Yamamoto; Hiroshi Shimada; Masao Fujita; Takeshi Kawasaki; Takuro Maruyama; Yukihiro Goda; Hajime Mizukami;

  • 作者单位

    Graduate School of Pharmaceutical Sciences Nagoya City University 3-1 Tanabe-dori Mizuho-ku Nagoya 467-8603 Japan;

    Pharmacognosy and Medicinal Resources Research Department Central Research Laboratories Tsumura ampamp Co. Ibaraki 300-119 Japan;

    Pharmacognosy and Medicinal Resources Research Department Central Research Laboratories Tsumura ampamp Co. Ibaraki 300-119 Japan;

    Tochimoto tenkaido Co. Oniya Kaibara Tanba Hyogo 669-3315 Japan;

    Tochimoto tenkaido Co. Oniya Kaibara Tanba Hyogo 669-3315 Japan;

    Uchida Wakanyaku Co. 4-4-1 Higashinippori Arakawa-ku Tokyo 116-8571 Japan;

    Uchida Wakanyaku Co. 4-4-1 Higashinippori Arakawa-ku Tokyo 116-8571 Japan;

    Division of Pharmacognosy Phytochemistry and Narcotics National Institute of Health Sciences 1-18-1 Kamiyoga Setagaya-ku-Tokyo 158-8501 Japan;

    Division of Pharmacognosy Phytochemistry and Narcotics National Institute of Health Sciences 1-18-1 Kamiyoga Setagaya-ku-Tokyo 158-8501 Japan;

    Graduate School of Pharmaceutical Sciences Nagoya City University 3-1 Tanabe-dori Mizuho-ku Nagoya 467-8603 Japan;

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  • 正文语种 eng
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  • 关键词

    DNA authentication of crude drug; Atractylodes lancea rhizome; Atractylodes rhizome; 18S–5.8S rDNA internal transcribed spacer sequence;

    机译：粗药物的DNA鉴定;白术;白术;18S–5.8S rDNA内部转录间隔区序列;

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