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首页> 外文期刊>Journal of Oleo Science >Novel Pre-fractionation Method of Trans Fatty Acids by Gas Chromatography with Silver-Ion Cartridge Column
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Novel Pre-fractionation Method of Trans Fatty Acids by Gas Chromatography with Silver-Ion Cartridge Column

机译：银离子柱色谱气相色谱法分离反式脂肪酸的新方法

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摘要

Abstract: We developed a novel pre-separation method of trans fatty acids (TFAs) using a silver-ion cartridge column and GC. As a preliminary study, a mixture of fatty acid methyl esters consisting of saturated, m-unsaturated, and frans-unsaturated fatty acids was dissolved in dichloromethane and loaded onto a Bond Elut SCX ion-exchange cartridge column that was converted to the silver-ion form. The column was then eluted with dichloromethane to obtain the saturated fatty acids, dichloromethane/ethyl acetate (90/10) for the trans mono-ene, dichloromethane/ethyl acetate (65/35) for the cis mono-ene, dichloromethane/ acetone (60/40) for the trans di-ene, and acetone/acetonitrile (80/20) for the others. Satisfactory separation of the cisltrans isomers was confirmed by GC analysis. To generalize this technique, the elution conditions of the ready-to-use Discovery Ag-ION SPE cartridge column were also optimized. Both cartridge columns had good separation, recovery, and repeatability. Peer laboratory verification was carried out between two laboratories using different production lots of the ready-to-use cartridge column, and the robustness of the product and reproducibility of the method were found to be satisfactory. This technique is therefore a powerful tool not only for routine analyses of TFAs in oils, fats, and foods but also for detailed analyses of TFAs in various research fields.
机译：摘要：我们开发了一种使用银离子柱和GC的反式脂肪酸（TFA）预分离的新方法。作为初步研究，将由饱和，间不饱和和反式氟烷不饱和脂肪酸组成的脂肪酸甲酯混合物溶解在二氯甲烷中，并加载到Bond Elut SCX离子交换柱上，该柱被转化为银离子形成。然后将柱子用二氯甲烷洗脱，得到饱和脂肪酸：反式单烯的二氯甲烷/乙酸乙酯（90/10），顺式单烯的二氯甲烷/丙酮（二氯甲烷/乙酸乙酯（65/35）（反式二烯为60/40），其他为丙酮/乙腈（80/20）。通过GC分析证实了顺反异构体的满意分离。为了推广该技术，还优化了即用型Discovery Ag-ION SPE柱的洗脱条件。两个小柱均具有良好的分离，回收率和可重复性。在两个实验室之间使用不同生产量的即用型柱色谱柱进行了同行实验室验证，结果发现该产品的耐用性和方法的可重复性令人满意。因此，该技术不仅是常规分析油脂，食品中TFA的有力工具，而且还是各种研究领域中TFA的详细分析的有力工具。

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  • 来源
    《Journal of Oleo Science》 |2012年第2期|p.49-56|共8页
  • 作者

    Hirofumi Goto; Noriko Shionoya; Megumi Sugie; Makoto Tominaga; Olga Shimelis; Makoto Taniguchi; Tomoji Igarashi; Yoshiaki Hirata;

  • 作者单位

    Japan Food Research Laboratories, Nagoya Branch (4-5-13 Osu, Naka-ku, Nagoya, Aichi 460-0011, JAPAN),Mimasaka University (50 Kitazono-cho, Tsuyama, Okayama 708-8511, JAPAN);

    Japan Food Research Laboratories, Nagoya Branch (4-5-13 Osu, Naka-ku, Nagoya, Aichi 460-0011, JAPAN);

    Japan Food Research Laboratories, Nagoya Branch (4-5-13 Osu, Naka-ku, Nagoya, Aichi 460-0011, JAPAN);

    Japan Food Research Laboratories, Nagoya Branch (4-5-13 Osu, Naka-ku, Nagoya, Aichi 460-0011, JAPAN);

    Supelco (595 North Harrison Road, Bellefonte, PA 16823, US);

    Mimasaka University (50 Kitazono-cho, Tsuyama, Okayama 708-8511, JAPAN);

    Japan Food Research Laboratories, Nagoya Branch (4-5-13 Osu, Naka-ku, Nagoya, Aichi 460-0011, JAPAN);

    Japan Food Research Laboratories, Nagoya Branch (4-5-13 Osu, Naka-ku, Nagoya, Aichi 460-0011, JAPAN);

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  • 正文语种 eng
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  • 关键词

    trans fatty acid; pre-fractionation; gas chromatography; silver-ion chromatography;

    机译：反式脂肪酸预分级气相色谱法;银离子色谱;

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