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首页> 外文期刊>Journal of Parasitic Diseases >Kinetics and time dependence of the differential agglutination of acetone [AC]- and formalin [HS]-fixed Toxoplasma tachyzoites by serum of mice with experimental toxoplasmosis
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Kinetics and time dependence of the differential agglutination of acetone [AC]- and formalin [HS]-fixed Toxoplasma tachyzoites by serum of mice with experimental toxoplasmosis

机译：弓形虫病小鼠血清中丙酮[AC]-福尔马林[HS]固定的弓形虫速殖子的差异凝集的动力学和时间依赖性

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摘要

Researches to specify a serologic diagnostic test capable of determining the stage of toxoplasmosis, whether recent or latent, have been hampered by lack of knowing the real time of infection. Studying the precise kinetics of the differential agglutination of acetone [AC]-fixed versus that of formalin [HS]-fixed tachyzoites (differential agglutination test or AC/HS test) by sera of mice during the course of toxoplasmosis and assessment of its value in the differentiation between recent and latent infections in mice were the aims of the present work. Experimental toxoplasmosis was induced in mice, sera were collected sequentially and AC/HS test, FAST-ELISA to determine levels of IgM and IgG and microscopic examination of brain for Toxoplasma cysts were done. Both AC and HS specific patterns in the AC/HS test were noted to be dependent on the time from the onset of infection. Acute patterns of the AC/HS test were observed early in infection till before the appearance of brain cysts. Non-acute patterns were obtained late on 28th day post infection coinciding with the disappearance of IgM, persistence of IgG and presence of cysts in brains. The AC antibody was high in the recent phase of infection, and then it declined to be replaced by high sustained level of HS antibody late in infection. In conclusion, in the presence of both IgM and IgG, the appearance of either equivocal pattern or the non-acute pattern in the AC/HS test is significant in ruling out acute infection in mice.
机译：由于缺乏对感染实时性的了解，阻碍了血清学诊断测试的研究能够确定弓形虫病的阶段，无论是近期的还是潜在的。研究弓形虫病过程中小鼠血清丙酮[AC]固定与福尔马林[HS]固定速殖子的差异凝集的精确动力学（差异凝集试验或AC / HS试验），并评估其在弓形虫病中的价值小鼠近期感染与潜伏感染之间的区别是本研究的目标。在小鼠中诱发实验性弓形虫病，顺序收集血清并进行AC / HS测试，FAST-ELISA测定IgM和IgG的水平，并对脑进行弓形虫囊肿的显微镜检查。注意到AC / HS测试中的AC和HS特定模式均取决于感染开始的时间。在感染早期至脑囊肿出现之前观察到了AC / HS测试的急性模式。在感染后第28天晚些时候获得了非急性模式，这与IgM的消失，IgG的持续存在和脑中囊肿的出现相吻合。 AC抗体在感染的最近阶段很高，然后在感染后期拒绝被高水平的HS抗体替代。总之，在同时存在IgM和IgG的情况下，AC / HS测试中模棱两可的模式或非急性模式的出现对于排除小鼠的急性感染很重要。

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