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首页> 外文期刊>Journal of Parasitology >DEVELOPMENT OF AN ENZYME-LINKED IMMUNOELECTROTRANSFER BLOT (EITB) ASSAY USING TWO BACULOVIRUS EXPRESSED RECOMBINANT ANTIGENS FOR DIAGNOSIS OF TAENIA SOLIUM TAENIASIS
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DEVELOPMENT OF AN ENZYME-LINKED IMMUNOELECTROTRANSFER BLOT (EITB) ASSAY USING TWO BACULOVIRUS EXPRESSED RECOMBINANT ANTIGENS FOR DIAGNOSIS OF TAENIA SOLIUM TAENIASIS

机译：用两种表达杆状病毒的重组杆状病毒来开发酶联免疫微转移印迹法（EITB）。

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Taeniasis diagnosis is an important step in the control and elimination of both cysticercosis and taeniasis. We report the development of 2 serological taeniasis diagnostic tests using recombinant antigens rES33 and rES38 expressed by baculovirus in insect cells in an EITB format. In laboratory testing with defined sera from nonendemic areas, rES33 has a sensitivity of 98% (n = 167) and a specificity of 99% (n = 310) (J index: 0.97); rES38 has a sensitivity of 99% (n = 146) and a specificity of 97% (n = 275) (J index: 0.96). Independent field testing in Peru showed 97% (n = 203) of the taeniasis sera were positive with rES33, and 100% of the nontaeniasis sera (n = 272) were negative with rES33; 98% (n = 198) of taeniasis sera were positive with rES38, and 91% (n = 274) of the nontaeniasis sera were negative with rES38. Among the Peruvian sera tested, 17 of 26 Peruvian Taenia saginata sera were false positive with rES38 test. Both tests were also examined with cysticercosis sera, with a positive rate ranging from 21% to 46%. rES33 and rES38 tests offer sensitive and specific diagnosis of taeniasis and easy sample collection through finger sticks that can be used in large-scale studies. They are currently being used in cysticercosis elimination programs in Peru.
机译：en虫病诊断是控制和消除囊尾rc病和and虫病的重要步骤。我们报告使用杆状病毒在EITB格式的昆虫细胞中表达的重组抗原rES33和rES38使用重组抗原rES33和rES38进行2种血清学虫病诊断测试的发展。在实验室检测非流行地区的血清时，rES33的敏感性为98％（n = 167），特异性为99％（n = 310）（J指数：0.97）； rES38的敏感性为99％（n = 146），特异性为97％（n = 275）（J指数：0.96）。在秘鲁进行的独立现场测试显示，rES33阳性的虫病血清为97％（n = 203），而rES33阴性的非ta虫病血清为100％（n = 272）； ES虫病血清中98％（n = 198）的rES38呈阳性，而非ta虫病血清中91％（n = 274）的rES38呈阴性。在秘鲁血清中，通过rES38检测，秘鲁26株牛带en虫中有17例为假阳性。两种测试还用囊尾rc病血清进行了检查，阳性率为21％至46％。 rES33和rES38测试可对带状疱疹进行灵敏而特异的诊断，并且可通过可用于大规模研究的手指棒轻松采集样品。它们目前在秘鲁用于消除囊尾rc病的计划中。

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