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首页> 外文期刊>Journal of Parasitology >Development of an enzyme-linked immunoelectrotransfer blot (EITB) assay using two baculovirus expressed recombinant antigens for diagnosis of Taenia solium taeniasis.
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Development of an enzyme-linked immunoelectrotransfer blot (EITB) assay using two baculovirus expressed recombinant antigens for diagnosis of Taenia solium taeniasis.

机译：开发了一种使用两种杆状病毒表达的重组抗原的酶联免疫电转移印迹（EITB）检测试剂盒，用于诊断Ta虫Ta虫病。

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摘要

Taeniasis diagnosis is an important step in the control and elimination of both cysticercosis and taeniasis. We report the development of 2 serological taeniasis diagnostic tests using recombinant antigens rES33 and rES38 expressed by baculovirus in insect cells in an EITB format. In laboratory testing with defined sera from nonendemic areas, rES33 has a sensitivity of 98% (n = 167) and a specificity of 99% (n = 310) (J index: 0.97); rES38 has a sensitivity of 99% (n = 146) and a specificity of 97% (n = 275) (J index: 0.96). Independent field testing in Peru showed 97% (n 203) of the taeniasis sera were positive with rES33, and 100% of the nontaeniasis sera (n = 272) were negative with rES33; 98% (n = 198) of taeniasis sera were positive with rES38, and 91% (n = 274) of the nontaeniasis sera were negative with rES38. Among the Peruvian sera tested, 17 of 26 Peruvian Taenia saginata sera were false positive with rES38 test. Both tests were also examined with cysticercosis sera, with a positive rate ranging from 21% to 46%. rES33 and rES38 tests offer sensitive and specific diagnosis of taeniasis and easy sample collection through finger sticks that can be used in large-scale studies. They are currently being used in cysticercosis elimination programs in Peru.
机译：en虫病诊断是控制和消除囊尾rc病和and虫病的重要步骤。我们报告了使用杆状病毒在昆虫细胞中以EITB格式表达的重组抗原rES33和rES38进行的2种血清学性en虫病诊断测试的发展。在实验室检测的非流行地区血清中，rES33的敏感性为98％（n = 167），特异性为99％（n = 310）（J指数：0.97）; rES38的敏感性为99％（n = 146），特异性为97％（n = 275）（J指数：0.96）。在秘鲁进行的独立现场测试显示，rES33阳性的97虫病血清为97％（203例），rES33阴性的非ta虫病血清为100％（n = 272）; ES虫病血清中98％（n = 198）与rES38呈阳性反应，非non虫病血清中91％（n = 274）与rES38呈阴性。在秘鲁血清中，通过rES38检测，秘鲁26株牛带Ta虫中有17例为假阳性。两种测试还检查了囊尾rc病血清的阳性率，范围从21％到46％。 rES33和rES38测试可对带状疱疹进行灵敏而特异的诊断，并且可通过可用于大规模研究的指尖轻松采集样品。它们目前正在秘鲁的消除囊尾病计划中使用。

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