A SMALL HEAT SHOCK PROTEIN OF OSTERTAGIA OSTERTAGI: STAGE-SPECIFIC EXPRESSION, HEAT INDUCIBILITY, AND PROTECTION TRIAL

Isabel Vercauteren Veerle De Maere Jozef Vercruysse Mieke Stevens Kris Gevaert and Edwin Claerebout

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A SMALL HEAT SHOCK PROTEIN OF OSTERTAGIA OSTERTAGI: STAGE-SPECIFIC EXPRESSION, HEAT INDUCIBILITY, AND PROTECTION TRIAL

机译：Ostertagia Ostertagi的一种小热激蛋白：特定阶段的表达，热诱导性和保护试验

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In this study, we isolated and analyzed a small heat shock protein (HSP) of Ostertagia ostertagi (Oo-HSP18). Oo-hsp18 is encoded by a single-copy gene and the full-length cDNA represents an 18-kDa protein. The expression of Oo-hsp18 is highly stage specific and restricted to the adult stage. The protein is synthesized in a tissue-specific manner and localized in the body muscle layer. The levels of Oo-hsp18 mRNAs are sharply induced by heat shock but not by other stressors such as levamisole and H2O2. A vaccination trial with recombinant Oo-HSP18 failed to protect calves against a challenge infection.

机译：在这项研究中，我们分离并分析了一种小型的Ostertagia ostertagi（Oo-HSP18）热休克蛋白（HSP）。 Oo-hsp18由单拷贝基因编码，全长cDNA代表18-kDa蛋白。 Oo-hsp18的表达具有高度的阶段特异性，并且仅限于成年阶段。该蛋白质以组织特异性方式合成，并位于人体肌肉层中。 Oo-hsp18 mRNA的水平是由热激强烈诱导的，而不是由诸如左旋咪唑和过氧化氢之类的其他应激源诱导的。重组Oo-HSP18的疫苗接种试验未能保护小牛免受挑战性感染。

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来源
《Journal of Parasitology》 |2006年第6期|p.1244-1250|共7页
作者
Isabel Vercauteren Veerle De Maere Jozef Vercruysse Mieke Stevens Kris Gevaert and Edwin Claerebout;
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作者单位

Laboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan, 133, B-9820 Merelbeke, Belgium. E-mail: veerle.demaere@ugent.be;

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关键词
heat shock protein,Ostertagia ostertagi,Ostertagia ostertagi;

机译：热休克蛋白;Ostertagia ostertagi;Ostertagia ostertagi;

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A SMALL HEAT SHOCK PROTEIN OF OSTERTAGIA OSTERTAGI: STAGE-SPECIFIC EXPRESSION, HEAT INDUCIBILITY, AND PROTECTION TRIAL

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