BINDING PROPERTIES AND IMMUNOLOCALIZATION OF A FATTY ACID–BINDING PROTEIN IN GIARDIA LAMBLIA

S. M T. Hassan M. Maache R. Díaz de la Guardia O. M. Córdova J. R. Gil García M. Galiana D. Acuña Castroviejo M. Martins* and Antonio Osuna†

首页> 外文期刊>Journal of Parasitology >BINDING PROPERTIES AND IMMUNOLOCALIZATION OF A FATTY ACID–BINDING PROTEIN IN GIARDIA LAMBLIA

【24h】

BINDING PROPERTIES AND IMMUNOLOCALIZATION OF A FATTY ACID–BINDING PROTEIN IN GIARDIA LAMBLIA

机译：贾第鞭毛虫中脂肪酸结合蛋白的结合特性及其免疫原化

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

We describe here a fatty acid–binding protein (FABP) isolated and purified from the parasitic protozoon Giardia lamblia. The protein has a molecular mass of 8 kDa and an isoelectric point of 4.96. A Scatchard analysis of the data at equilibrium revealed a dissociation constant of 3.12 × 10−8 M when the labeled oleic acid was displaced by a 10-fold greater concentration of unlabeled oleic acid. Testosterone, sodium desoxycholate, taurocholate, metronidazol, and α-tocopherol, together with butyric, arachidonic, palmitic, retinoic, and glycocholic acids, were also bound to the protein. Assays with polyclonal antibodies revealed that the protein is located in the ventral disk and also appears in the dorsal membrane, the cytoplasm, and in the vicinity of the lipid vacuoles.

机译：我们在这里描述了一种从寄生原生动物贾第鞭毛虫中分离和纯化的脂肪酸结合蛋白（FABP）。该蛋白质的分子量为8 kDa，等电点为4.96。平衡状态下数据的Scatchard分析显示，当标记的油酸被未标记的油酸浓度提高10倍时，解离常数为3.12×10-8M。睾丸激素，脱氧胆酸钠，牛磺胆酸盐，甲硝唑和α-生育酚，以及丁酸，花生四烯酸，棕榈酸，视黄酸和糖胆酸也都与该蛋白质结合。用多克隆抗体的分析表明该蛋白位于腹盘中，并且也出现在背膜，细胞质和脂质液泡附近。

著录项

来源
《Journal of Parasitology》 |2005年第2期|p.00000284-00000292|共9页
作者
S. M T. Hassan M. Maache R. Díaz de la Guardia O. M. Córdova J. R. Gil García M. Galiana D. Acuña Castroviejo M. Martins* and Antonio Osuna†;
展开▼
作者单位

Instituto de Biotecnología, Universidad de Granada, Campus Universitario de Fuentenueva, E-18071 Granada, Spain. aosuna@ugr.es*Fundação de Medicina Tropical-FMT/IMT-AM, Gerência de Parasitologia, Manaus-AM CEP: 69040 000, Brazil†To whom correspondence should be addressed;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类
关键词

相似文献

外文文献
中文文献
专利

1. PURIFICATION AND CHARACTERIZATION OF A PROTEIN CAPABLE OF BINDING TO FATTY ACIDS AND BILE SALTS IN GIARDIA LAMBLIA [J] . Diaz de la Guardia R., Lopez M. B., Burgos M., Journal of Parasitology . 2011,第4期

机译：贾第鞭毛虫中能与脂肪酸和胆盐结合的蛋白质的纯化和鉴定
2. Studies on fatty acid-binding proteins. The binding properties of rat liver fatty acid-binding protein [J] . D C Wilton, T C Wilkinson The biochemical journal . 1987,第2期

机译：脂肪酸结合蛋白的研究。大鼠肝脏脂肪酸结合蛋白的结合特性
3. Gene/protein expression level, immunolocalization and binding characteristics of fatty acid binding protein from Clonorchis sinensis (CsFABP) [J] . HuangL., HuY., HuangY., Molecular and Cellular Biochemistry: An International Journal for Chemical Biology . 2012,第1a2期

机译：华支睾吸虫脂肪酸结合蛋白（CsFABP）的基因/蛋白表达水平，免疫定位和结合特性
4. THE ROLE OF EXTRACELLULAR FATTY ACID-BINDING PROTEINS IN CELLULAR TRANSPORT OF FATTY ACIDS [C] . Judith Storch, Fiona M. Herr, Kuo Tung Hsu, International Washington Spring Symposium . 1996

机译：细胞外脂肪酸结合蛋白在脂肪酸细胞转运中的作用
5. Functional characterisation of a TATA binding protein (TBP) and a TBP interacting protein (TIP49a) in Giardia lamblia. [D] . Joseph, Joella. 2010

机译：贾第鞭毛虫中的TATA结合蛋白（TBP）和TBP相互作用蛋白（TIP49a）的功能表征。
6. Human Secretory Immune Response to Fatty Acid-Binding Protein Fraction from Giardia lamblia [O] . S. M. T. Hasan, M. Maachee, O. M. Córdova, 2002

机译：贾第鞭毛虫对脂肪酸结合蛋白组分的人体分泌免疫反应
7. Human Secretory Immune Response to Fatty Acid-Binding Protein Fraction from Giardia lamblia [O] . Hasan, S. M. T., Maachee, M., Córdova, O. M., 2002

机译：贾第鞭毛虫对脂肪酸结合蛋白组分的人体分泌免疫反应

BINDING PROPERTIES AND IMMUNOLOCALIZATION OF A FATTY ACID–BINDING PROTEIN IN GIARDIA LAMBLIA

摘要

著录项

相似文献

相关主题

期刊订阅