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首页> 外文期刊>Journal of Parasitology >Suppressed Cytokine and Immunoglobulin Secretions by Murine Splenic Lymphocytes Infected In Vitro with Toxoplasma gondii Tachyzoites
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Suppressed Cytokine and Immunoglobulin Secretions by Murine Splenic Lymphocytes Infected In Vitro with Toxoplasma gondii Tachyzoites

机译：弓形虫速殖子体外感染的小鼠脾脏淋巴细胞抑制细胞因子和免疫球蛋白分泌

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摘要

Mechanisms of host immunosuppression after infection with Toxoplasma gondii are unclear. This study was performed to observe cytokine and immunoglobulin secretions by murine splenic lymphocytes infected in vitro with live, nonreplicating (irradiated) RH tachyzoites on stimulation with concanavalin A (Con A) or lipopolysaccharide (LPS). For lymphocyte cultivation, 3 groups were prepared: coculture with live nonirradiated tachyzoites separated by a transwell (group T), live irradiated tachyzoites without a transwell (group R), and no tachyzoites (group C). Compared with group T, groups R and C, on stimulation with Con A, revealed significantly (P < 0.05) lower levels of interleukin-2 (IL-2) and IFN-γ, but not IL-10. The levels of IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM were also significantly (P < 0.05) lower in groups R and C than in group T after stimulation with LPS. The results suggest that intracellular infection of murine splenic lymphocytes with T. gondii tachyzoites could impair their capacity to produce cytokine and immunoglobulin secretions.
机译：弓形虫感染后宿主免疫抑制的机制尚不清楚。进行这项研究的目的是观察在体外感染了伴刀豆球蛋白A（Con A）或脂多糖（LPS）的活的，非复制的（辐照）RH速殖子感染的小鼠脾脏淋巴细胞中细胞因子和免疫球蛋白的分泌。为了进行淋巴细胞培养，准备了3个组：与被跨孔分开的未辐照的活速殖子共培养（T组），没有跨孔的活辐照的速殖子（R组）和没有速殖子（C组）。与T组相比，R和C组在用Con A刺激后，显着（P <0.05）降低了白介素2（IL-2）和IFN-γ的水平，但没有降低IL-10。用LPS刺激后，R和C组的IgG1，IgG2a，IgG2b，IgG3，IgA和IgM的水平也显着降低（P <0.05）。结果表明，刚地弓形虫速殖子对鼠脾淋巴细胞的细胞内感染可能损害它们产生细胞因子和免疫球蛋白分泌的能力。

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  • 来源
    《Journal of Parasitology》 |2005年第2期|p.00000467-00000470|共4页
  • 作者

    S-M. Guka J. Kookab Y-H. Jeonac J-H. Choiad E-T. Hanae E-H. Shina and J-Y. Chaiaf;

  • 作者单位

    aDepartment of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea bDepartment of Biochemical Engineering, Yanbian University of Science and Technology, Yanji City, Jilin Province 133000, P.R. China cDepartment of Pediatrics, Inha University College of Medicine, Inchon 400-711, Korea dDepartment of Pediatrics, Seoul National University Children's Hospital, Seoul 110-744, Korea eDepartment of Parasitology, Kangwon National University College of Medicine, Chunchon 200-701, Korea fTo whom correspondence should be addressed. cjy@snu.ac.kr;

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