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首页> 外文期刊>Journal of Parasitology >TRYPANOSOMA CRUZI I AND TRYPANOSOMA CRUZI II: RECOGNITION OF SUGAR STRUCTURES BY ARACHIS HYPOGAEA (PEANUT AGGLUTININ) LECTIN
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TRYPANOSOMA CRUZI I AND TRYPANOSOMA CRUZI II: RECOGNITION OF SUGAR STRUCTURES BY ARACHIS HYPOGAEA (PEANUT AGGLUTININ) LECTIN

机译：TRYPANOSOMA CRUZI I和TRYPANOSOMA CRUZI II：花生低聚糖（花生）凝集素对糖结构的识别

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摘要

Epimastigote culture forms of different isolates of Trypanosoma cruzi from different mammal hosts, humans, and vectors were tested with FITC-conjugated peanut agglutinin lectin (PNA–FITC). The parasites maintained in axenic medium, liver infusion tryptose, were evaluated by flow cytometric analyses; whereas T. cruzi I (TcI), which is associated with the sylvatic transmission cycle, was labeled in high percentages with PNA (88–99.2%), T. cruzi II (TcII) (parasites associated with domiciliar cycle) and T. cruzi, zymodeme 3 (Tc/Z3) (also associated with the sylvatic cycle) were labeled in low percentages (TcII, 0–26% and Tc/Z3, 0–12.6%). It was demonstrated that it is possible to differentiate the 2 main T. cruzi subpopulations, TcI and TcII, using Arachis hypogaea. These results also showed a higher variability in TcII in terms of PNA binding.
机译：用FITC偶联的花生凝集素凝集素（PNA-FITC）测试了来自不同哺乳动物宿主，人类和载体的克氏锥虫分离株的淫后鞭毛虫的培养形式。通过流式细胞仪分析评估了保持在轴突培养基中的寄生虫，即肝浸入类胰蛋白酶。而与Sylvatic传播周期相关的克鲁斯T. cruzi I（TcI）被PNA（88-99.2％），克鲁斯T. cruzi II（TcII）（与居家周期相关的寄生虫）和克鲁斯T. ，zymodeme 3（Tc / Z3）（也与sylvatic周期有关）被低百分比标记（TcII，0–26％和Tc / Z3，0–12.6％）。结果表明，有可能利用花生到花生来区分两个主要的克鲁氏锥虫亚群TcI和TcII。这些结果还表明，在TcII中，与PNA结合的变异性更高。

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    《Journal of Parasitology》 |2002年第3期|p.582-586|共5页
  • 作者

    C. A C. Araújo C. B. Mello* A. M. Jansen;

  • 作者单位

    Department of Protozoology, Instituto Oswaldo Cruz, FIOCRUZ, Avenue Brasil 4365, Rio de Janeiro, Manguinhos, Brazil 21045-900. jansen@ioc.fiocruz.br;

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