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Comparable Growth of Tritrichomonas mobilensis in Two Commercially Available Culture Media

机译：在两种市售培养基中Tritrichomonas mobilensis的可比增长

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摘要

The investigation reported herein was undertaken to determine which medium is more practical for the axenic laboratory culture of trichomonads. The growth of Tritrichomonas mobilensis was monitored in 2 different types of commercially available growth media. Although Roswell Park Memorial Institute (RPMI) 1640 medium is typically used as a mammalian cell culture medium, it was found to support the growth of trichomonads as well as the American Type Culture Collection (ATCC) medium 745 under similar conditions. Environmental variables, such as temperature and pH, known to affect the success of trichomonad cultures were controlled. The mean generation times (MGTs) of T. mobilensis in the log phase of growth were 5.1 and 4.9 hr for RPMI 1640 and ATCC medium 745, respectively. A stationary phase of zero growth was reached more quickly in the ATCC medium 745 cultures, and in both media a phase of rapid attrition followed this period of static growth. In assessing the practicality of the media, total cell amplification, as well as factors such as cost, ease of preparation, and storage capacity, were considered.
机译：进行本文报道的研究以确定哪种培养基更适合滴虫的无菌实验室培养。在两种不同类型的市售生长培养基中监测了Tritrichomonas mobilensis的生长。尽管罗斯威尔公园纪念学院（RPMI）1640培养基通常用作哺乳动物细胞培养基，但发现它在相似条件下支持滴虫和美国典型培养物保藏中心（ATCC）培养基745的生长。控制已知影响滴虫培养成功的环境变量，例如温度和pH。对于RPMI 1640和ATCC培养基745，在生长的对数生长期中，T。mobilensis的平均产生时间（MGT）分别为5.1小时和4.9小时。在ATCC 745培养基中，生长更快地达到了零生长的稳定阶段，在这两种培养基中，此静态生长阶段之后都是快速损耗的阶段。在评估培养基的实用性时，考虑了总细胞扩增以及诸如成本，易于制备和储存容量等因素。

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    《Journal of Parasitology》 |2002年第5期|p.1012-1014|共3页
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    Andrea K. Boggilda Christine A. Sundermannb Barbara H. Estridgeb and David S. Lindsayc;

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    aRoom 2124 Medical Sciences Building, 1 King's College Circle, Faculty of Medicine, University of Toronto, Ontario, Canada M5S 1A8. andrea.boggild@utoronto.ca bDepartment of Biological Sciences, 131 Cary Hall, Auburn University, Alabama 36849 cDepartment of Biomedical Sciences and Pathobiology, Virginia–Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia 24061-0342;

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