COMPARISON OF CONVENTIONAL TECHNIQUES TO DIFFERENTIATE BETWEEN TAENIA SOLIUM AND TAENIA SAGINATA AND AN IMPROVED POLYMERASE CHAIN REACTION-RESTRICTION FRAGMENT LENGTH POLYMORPHISM ASSAY USING A MITOCHONDRIAL 12S rDNA FRAGMENT

R. Rodriguez-Hidalgo D. Geysen* W. Benítez-Ortiz S. Geerts† and J. Brandt†

首页> 外文期刊>Journal of Parasitology >COMPARISON OF CONVENTIONAL TECHNIQUES TO DIFFERENTIATE BETWEEN TAENIA SOLIUM AND TAENIA SAGINATA AND AN IMPROVED POLYMERASE CHAIN REACTION-RESTRICTION FRAGMENT LENGTH POLYMORPHISM ASSAY USING A MITOCHONDRIAL 12S rDNA FRAGMENT

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COMPARISON OF CONVENTIONAL TECHNIQUES TO DIFFERENTIATE BETWEEN TAENIA SOLIUM AND TAENIA SAGINATA AND AN IMPROVED POLYMERASE CHAIN REACTION-RESTRICTION FRAGMENT LENGTH POLYMORPHISM ASSAY USING A MITOCHONDRIAL 12S rDNA FRAGMENT

机译：TA虫SOL虫和SA虫EN虫与改良的聚合酶链反应-限制性片段长度多态性分析的常规技术比较（使用线粒体12S rDNA片段进行比较）

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Given the constraints of classical diagnostic methods, i.e., morphological and isoenzymatic studies of proglottids, a polymerase chain reaction test complemented with restriction enzyme analysis has been modified by redesigning one of the primers to reduce nonspecific amplifications experienced when using field samples. The use of these new, highly cestode-specific primers and the restriction enzyme DdeI led to the development of a diagnostic assay that clearly distinguishes between Taenia saginata and T. solium proglottids in field samples. This assay confirms the presence of T. saginata in Ecuador. DNA amplification of some of these taeniids showed different patterns, suggesting the possibility that strain differences exist. These results demonstrate the need for development of useful molecular assays as reliable tools for epidemiological studies on cestodes.

机译：考虑到经典诊断方法的局限性，即对proglottids的形态学和同工酶研究，通过重新设计其中一种引物以减少使用田间样品时遇到的非特异性扩增，对修饰有限制酶分析的聚合酶链反应测试进行了修改。使用这些新的，高度鲸蜡胚特异性引物和限制酶DdeI导致了诊断分析的发展，该诊断分析可以清楚地区分田Ta中的Ta虫和and虫。该测定法证实厄瓜多尔存在T. saginata。其中一些taeniids的DNA扩增显示出不同的模式，表明存在菌株差异的可能性。这些结果表明需要开发有用的分子测定方法作为对虫进行流行病学研究的可靠工具。

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《Journal of Parasitology》 |2002年第5期|p.1007-1011|共5页
作者
R. Rodriguez-Hidalgo D. Geysen* W. Benítez-Ortiz S. Geerts† and J. Brandt†;
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Laboratorio de Inmunodiagnostico e Investigación, Facultad de Medicina Veterinaria y Zootecnia, Universidad Central del Ecuador, Quito, Ecuador. dgeysen@itg.be;

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COMPARISON OF CONVENTIONAL TECHNIQUES TO DIFFERENTIATE BETWEEN TAENIA SOLIUM AND TAENIA SAGINATA AND AN IMPROVED POLYMERASE CHAIN REACTION-RESTRICTION FRAGMENT LENGTH POLYMORPHISM ASSAY USING A MITOCHONDRIAL 12S rDNA FRAGMENT

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